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Purple: H + -ATPase; blue: AUX1; dashed arrow: diffusion Yellow: PIN proteins ; red: PGPs

Purple: H + -ATPase; blue: AUX1; dashed arrow: diffusion Yellow: PIN proteins ; red: PGPs. PINs/PGP proteins:. Tissue-specific distribution of PIN efflux facilitator proteins. br2 mutant:. Normal tassels and ear, but compact lower stalks. Auxin transport inhibitors. Aglycone flavonoids.

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Purple: H + -ATPase; blue: AUX1; dashed arrow: diffusion Yellow: PIN proteins ; red: PGPs

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  1. Purple: H+-ATPase;blue: AUX1; dashed arrow: diffusion Yellow: PIN proteins; red: PGPs

  2. PINs/PGP proteins: Tissue-specific distribution of PIN efflux facilitator proteins

  3. br2 mutant: Normal tassels and ear, but compact lower stalks

  4. Auxin transport inhibitors Aglycone flavonoids efflux inhibitor isoflavones influx inhibitor displace NPA

  5. Actin-dependent PIN complex cycling PLoS ONE (January 2008) In dark: PIN2, in vacuole; In light: predominantly in PM Auxin transport inhibitors may act by interfering with PIN1 complex cycling Web Essay 19.1

  6. Physiological effects of auxin –cell elongation Oat coleoptile sections Endogenous vs. Exogenous auxin Suc maintain turgor pressure; can be substituted by KCl; 15 min lag time

  7. The optimal auxin concentration– pea stem or oat coleoptile sections (ethylene induction) ppm For roots: opt. 10-10 to 10-9 M; 10-6 M inhibition Not only for IAA level but also for auxin types for different cells

  8. 10 M IAA, 2% sucrose The characteristics of auxin induce stem growth: acquire energy is inhibited when the presence of inhibitor of metabolic, protein synthesis (cycloheximide), or RNA synthesis the limiting time of lag phase could not be shortened  somebiochemical machinery involved 0.5 h

  9. Auxin-induced elongation vs. cell wall acidification Maize coleoptile lag time

  10. Current models for IAA-induced H+ extrusion Receptor (?) p. 503 polyubiquitin ABP1: track H+-ATPase to membrane (auxin-binding protein) (cell wall loosening proteins)

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