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Progress tomato chromosome 6 René Klein Lankhorst

Progress tomato chromosome 6 René Klein Lankhorst. 155 BACs sequenced (64 seeds plus 91 extension BACs), 140 BACs arranged into 12 major contigs Short arm completely covered 3 small gaps remain Long arm has 5 major gaps left 16 Mb of BAC DNA sequenced from the euchromatin

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Progress tomato chromosome 6 René Klein Lankhorst

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  1. Progress tomato chromosome 6René Klein Lankhorst

  2. 155 BACs sequenced (64 seeds plus 91 extension BACs), 140 BACs arranged into 12 major contigs Short arm completely covered 3 small gaps remain Long arm has 5 major gaps left 16 Mb of BAC DNA sequenced from the euchromatin These BACs assembled into 12 Mb unique DNA Total euchromatine in chr.# 6 is approx. 20 Mb  60% done 76 BACs available on SGN 69 Phase 1 /2 8 Phase 3 Status chromosome #6 TG193

  3. 3 novel seed BACs resulted from a targeted screening of the MboI BAC library with overgo markers (Joyce van Eck) 21 AFLP markers plus 10 SNP markers have been selected for the different gaps (Keygene). Currently, 16 BACs have been retrieved with these markers Targeting the gaps on the long arm

  4. GAP closure of chr.#6 BACs • 130 Phase 1/2 BACs are/were in the finishing pipeline • In total, more than 800 gaps were present in these 130 BACs (average ~6 gaps / BAC) • In the past 6 months ~400 gaps have been closed resulting in the finishing of 37 BACs.

  5. The Dutch chromosome 6 sequencing team Sequence providers: • Sander Peters • Marleen Henkens • Marjo van Staveren • José van der Belt • Dorá Szinay • Erwin Datema • Yulin Bai • Roeland van Ham • Hans de Jong • Willem Stiekema • René Klein Lankhorst Funding:

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