1 PCR for DS13a2 is conducted at a primer annealing temperature of 53ºC.

Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Negative control Subject DNA Negative control Subject DNA Subject DNA Negative control Negative control Negative control Subject DNA Subject DNA Negative control Subject DNA Subject DNA Negative control Subject DNA Subject DNA Subject DNA Negative control Subject DNA Subject DNA Negative control Negative control Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Negative control Negative control Subject DNA Negative control Subject DNA Subject DNA Subject DNA Negative control Negative control Negative control Subject DNA Negative control Subject DNA Subject DNA Negative control Subject DNA Subject DNA Subject DNA Subject DNA Subject DNA Negative control Negative control Subject DNA Subject DNA Negative control Subject DNA Negative control DS17 DS10 DS15 DS16 DS12 DS10 DS16 DS11 DS11 DS11 DS12 DS12 DS26 DS10 DS25 DS20 DS24 DS142 DS20 DS22 DS21 DS21 DS20 DS25 DS17 DS35 DS17 DS22 DS23 DS23 DS24 DS21 DS142 DS29 DS23 DS30 DS32 DS34 DS26 DS24 DS36 DS29 DS142 DS33 DS29 DS34 DS32 DS33 DS30 DS36 DS25 DS30 DS35 DS15 DS16 DS26 DS22 DS31 DS36 DS15 DS34 DS35 DS31 DS32 DS33 DS31 Subject DNA Subject DNA Negative control DS13a21 DS13a21 DS13a21 Subject DNA Subject DNA Negative control DS13b DS13b DS13b PCR conditions: 94ºC for 15 min 40 cycles of following: 94ºC for 20 sec 58ºC for 30 sec1, 2 72ºC for 60 sec 72ºC for 3 min 1PCR for DS13a2 is conducted at a primer annealing temperature of 53ºC. 2PCR for DS14 is conducted at a primer annealing temperature of 62ºC. Supplementary Figure - PRNP PCRs in 96-well format.

1 PCR for DS13a2 is conducted at a primer annealing temperature of 53ºC.

1 PCR for DS13a2 is conducted at a primer annealing temperature of 53ºC.

Presentation Transcript

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