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細胞模型和生物晶片平台的發展 (The development of cell model and biochip platform)

細胞模型和生物晶片平台的發展 (The development of cell model and biochip platform). Prof. Ching-Hsing Luo National Cheng Kung University Tainan, Taiwan. Cell Model in Ion Channles Luo-Rudy model in 1991 &1994 Circ. Res. Ca 2+. Action Potential by Model Simulation.

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細胞模型和生物晶片平台的發展 (The development of cell model and biochip platform)

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  1. 細胞模型和生物晶片平台的發展(The development of cell model and biochip platform) Prof. Ching-Hsing Luo National Cheng Kung University Tainan, Taiwan

  2. Cell Model in Ion ChannlesLuo-Rudy model in 1991 &1994 Circ. Res. Ca2+

  3. Action Potential by Model Simulation Luo-Rudy model simulation by Prof. Ching-Hsing Luo • A standard procedure of model simulation has been developed to • evaluate the accuracy of simulation results • 2. A physiological simulation fake is discussed.

  4. International Invitation: Cell Attachment for Drug screen by Miqin Zhang High performance of integrated cell-based sensors for drug screening with impedance measurement technology.

  5. Mission • Medical Researches Stem cell, Neuron cell, Cancer cell for Drug screening, Differentiation or cultivation control, Gene therapy • Engineering Technologies Chip – micro/nano (channel and pumping) Sensor and actuator – micro/nano scale Cell Modeling – Ischemia, drug effects

  6. Team Up Domestic: • Cell-based microchip platform –李清庭、羅錦興、魏憲鴻、 鍾宜璋 • Cell Model - 吳勝男、羅錦興、鄧君豪 • Bio-application – 宋瑞珍副校長、林茂村院長、吳勝男 International: Univ. of Washington • Miqin Zhang: drug screening • Meldrum: HIV, AIDS applications

  7. Integration Structure Patch Clamping Microchip Platform with Cell Modeling (Project 3) Microfluidic Chip (Project 2) Single-Cell Microchip Operation Platform (protocol control, cell clamp, and cell model) Bio-application studies: stem cells, cardiac cells, and neurons (Project 1) Common Facilities (Core A) International and domestic collaboration

  8. Commercial Microarray chip

  9. Microfluid Flow Single Cell Microelectrode Micromixer Microchannel Microelectrode Microvalve Micropump Microvalve Flow sensor 1. Automatic Protocol control (concentration clamp) 2. Voltage clamp for ionic channel measurement Micropump Cell Chip Platform

  10. Passive Mixing in Microfluidic Channelby Prof. Wei q Channel Width Setup Width 300 mm Open angle q=60

  11. Mixing Results-1

  12. α q R Mixing Results-2

  13. Silicon oxide Differentiation or cultivation protocols Electrode Sucking pressure Silicon Glass Cell Clamping Microchip Platform

  14. Cell Electrode Channel Cell Attachment and Rejection Cell Rejection Compound

  15. PDMS Cell-Attachment (Implementation-1)by Prof. Chung and Prof. Luo • Using flat PDMS imprint SH-(CH2)11COOH on Au cell favoring coating Imprinting： Time≒ 60sec Chemical bond: SH-(CH2)11COOH Au

  16. Cell-Attachment (Implementation-2) • Immerse into DSPC solution (about 12~24 hours) • (cell hating) DSPC cell

  17. Cell-Attachment (Result-1)by Prof. Lin, Prof. Chung and Prof. Luo Morphology of the cell on the platform • Different geometric platform： square、circle、ellipse • Different size platform (all are 1.6 μm height)

  18. Preparations • Cell name : human CD34+ Progenitor Cell • Diluent : Phosphate Buffered Saline (PBS) • Na2HPO4 -------------------------------------- 10.9 g • NaH2PO4 -------------------------------------- 3.2 g • NaCl ------------------------------------------- 90 g • Distilled water --------------------------------- 1000 ml • Counterstain :Trypan blue

  19. Cell still can be adhered even platform is smaller than it Electrode smaller than cell 8x8 μm2 square platform 7x7 μm2 square platform

  20. Attachment Structure flowing Platform (a)Before adhering cell 1.6 μm silicon cell (b) After adhering platform Au on silicon base

  21. Electrode Shape Effect • Cell is stretched to platform corner • μmdiameter • circle platform 18x18 μm2 square platform 18x36 μm2 ellipse platform

  22. Spherical Shape Original platform cell Au on silicon base platform

  23. Rectangular Shape Original platform cell Au on silicon base platform

  24. Electrode bigger than cell • Cell may be stretched to platform edge 27x54 μm2 ellipse platform 37μmdiameter circle platform 37x74 μm2 ellipse platform 38μmdiameter circle platform

  25. Future Work • Microfluidic driving system • Voltage Clamp Chip (2-4 mm hole) • Protocol control interacts with ionic channel measurement • Update Cardiac Cell Model • Vascular Epithelial Cell model for Atherogenic analysis

  26. Thanks for Your Attention

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