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Advisor: Wei-Shou Hu Speaker: Justice Akhuamhenkhun Ace Lai

PRODUCTION OF 1,3-PROPANEDIOL THROUGH METABOLIC ENGINEERING. Advisor: Wei-Shou Hu Speaker: Justice Akhuamhenkhun Ace Lai. Properties of 1,3-PD. Molecular weight 76.09 Viscous and colorless liquid Miscible with water Density = 1.0597 g/ cm

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Advisor: Wei-Shou Hu Speaker: Justice Akhuamhenkhun Ace Lai

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  1. PRODUCTION OF 1,3-PROPANEDIOL THROUGH METABOLIC ENGINEERING • Advisor: Wei-Shou Hu • Speaker: Justice Akhuamhenkhun • Ace Lai

  2. Properties of 1,3-PD • Molecular weight 76.09 • Viscous and colorless liquid • Miscible with water • Density = 1.0597 g/ cm • MP = -28; BP, between 210 to 212 (celsius degree) • Unsaturated compound with hydroxyl functional group

  3. Applications • copolyesters, polymers, textile fibres, and carpets • organic solvent ingredient of wood paints and anti-freeze • chief ingredient manufacturing adhesives, laminates, terracota- as protective reagent

  4. Why we use bioengineering • Cheaper in cost • Easier in designing pathway • Greener to environment

  5. Metabolic pathway

  6. Metabolic engineering I • nature of synthesizing of 1,3PD • tpi gene • dhaT and dhaB, gene encoding glycerol dehydratase and PDOR • DAR1, GPP2 encoding 3-phosphate dehydrogenase and glycerol 3-phosphate phosphatase

  7. Metabolic engineering II • Redesigning of yqhD • Use of NADPH instead NADH • knock down of tpi to manipulate pathway more efficiently

  8. Calculation analysis • Yield, 1,3-propanediol at a rate of 3.5 g/L/h, a titer of 135 g/L, and a weight yield of 51% in D-glucosefed-batch 10 L fermentations.

  9. Conclusion • DAR1 and GPP2 cloned from Saccharamyces cerevisiae encoding 3-phosphate dehydrogenase • dhaB, dhaT gene from Klebsiella pneumoniae encoding PDOR and glycerol dehydratase • yqhD from E. coli can produce PDOR as well • tpi manipulated via knockdown to resolve carbon flux • PEP and ATP dependent glucose pathway modified • In all, contribute to higher yield synthesis

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