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Chapter 2: Sources of DNA Evidence. Forensic Biology by Richard Li. Introduction to Cells. DNA is located in cells which are the building blocks of the human body Two Types of cells: Sex Cells Somatic Cells. Introduction to Cells. Cell membrane Cytoplasm Nucleus.
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Chapter 2: Sources of DNA Evidence Forensic Biologyby Richard Li
Introduction to Cells • DNA is located in cells which are the building blocks of the human body • Two Types of cells: • Sex Cells • Somatic Cells Forensic Biology by Richard Li
Introduction to Cells • Cell membrane • Cytoplasm • Nucleus Forensic Biology by Richard Li
Examples of Biological Evidence • Blood • Most common type found at crime scenes • Consists of erythrocytes (red blood cells), leucocytes (white blood cells), and platelets • Nuclear DNA obtained from leucocytes Forensic Biology by Richard Li
Examples of Biological Evidence • Hair • Consists of root and shaft • Medulla • Cortex • Cuticle • Grows at approximately 0.3mm per day • Anagen phase- growing phase • Catagen phase- follicle becomes inactive • Telogen phase- growth cycle ends Forensic Biology by Richard Li
Examples of Biological Evidence • Hair as a source of DNA evidence • PCR allowed more productive analysis of hair • Formerly just analyzed morphology and comparisons • mtDNA can be isolated from roots and shafts • Nuclear DNA analysis or hair is preferred • Plucked hair with roots • Naturally shed hairs contain little nuclear DNA • Most hairs at crime scenes are naturally shed Forensic Biology by Richard Li
Examples of Biological Evidence • Bone • Compact vs. Spongy • Osteocytes • Osteoblasts • Osteoclasts Forensic Biology by Richard Li
Examples of Biological Evidence • Bone • Rate of degradation of human remains varies greatly with environmental conditions • Soft tissue is lost • Bones are more stable • Mass Fatalities-identify victims • Sanding- used to reduce potential of comingled remains, adhering inhibitors, and bacterial contamination Forensic Biology by Richard Li
Examples of Biological Evidence • Teeth • Deciduous/Primary teeth vs. Secondary dentition/Permanent dentition • Dentin- Does not contain cells • Pulp Cavity • Root Forensic Biology by Richard Li
Examples of Biological Evidence • Teeth • Identity of a person • Characteristics of teeth • Alignment • Overall structure • X-rays • Bite mark analysis Forensic Biology by Richard Li