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Forensic Biology by Richard Li

Chapter 7: Species Identification. Forensic Biology by Richard Li. General Considerations. Species Identification: In the past, done by serological methods Now, accomplished by DNA quantitation Can be a good screening method for unnecessary tests Can be used at the crime scene

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Forensic Biology by Richard Li

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  1. Chapter 7: Species Identification Forensic Biologyby Richard Li

  2. General Considerations • Species Identification: • In the past, done by serological methods • Now, accomplished by DNA quantitation • Can be a good screening method for unnecessary tests • Can be used at the crime scene • Necessary in animal cases • Use primary and secondary binding assays Forensic Biology by Richard Li

  3. Types of Antibodies • Antihuman serum • Albumin • Hemoglobin antibody • Glycophorin A Forensic Biology by Richard Li

  4. Titration of Antibodies • Series of dilutions of antiserum are made and each dilution is then tested for activity using either precipitation or agglutination methods • Titer- the reciprocal of the highest dilution giving a positive reaction. Forensic Biology by Richard Li

  5. Specificity • Most antihuman sera usually have cross-reactivity with higher primates • Crimes involving primates are rare Forensic Biology by Richard Li

  6. Optimal Conditions • Proper buffer system • Ionic strength • Intro of polymers • Temperature • pH Forensic Biology by Richard Li

  7. Assays • Immunochromatographic Assays • Identification of human hemoglobin protein • Hexagon OBTI • ABAcard HemaTrace • Identification of human glycophorin A protein • RSID-Blood Forensic Biology by Richard Li

  8. Assays • Precipitation-based assays • Ring assay • Ouchterlony assay • Identity • Non-identity • Partial identity • Crossed-over electrophoresis Forensic Biology by Richard Li

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