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pGEM -T

Gene of Interest. SalI NotI. NotI ApaI. pGEM -T. pGEM -T. NotI + ApaI. Amp-r. SalI + NotI. Amp-r. After 4 digestions, mix them all together & ligate Select for Kan-r & Gent-r Screen for Amp-s. SalI NotI NotI ApaI. sacB. Gent-r.

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pGEM -T

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  1. Gene of Interest SalI NotI NotI ApaI pGEM-T pGEM-T NotI + ApaI Amp-r SalI + NotI Amp-r After 4 digestions, mix them all together & ligate Select for Kan-r & Gent-r Screen for Amp-s SalI NotI NotI ApaI sacB Gent-r SalI ApaI Kan-r NotI NotI SalI + ApaI NotI pJQ200SK pCRS487 sacB Gent-r For more information, contact Ravi Tiwari at r.tiwari@murdoch.edu.au

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