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YEAST LAB

YEAST LAB. DEAD YEAST W/MINERAL OIL 2 mL of sucrose solution to 50 mL beaker Add 2 mL of DEAD yeast; pipette up and down 2-3x to mix Cover this solution with mineral oil Incubate beaker in warm water bath (38°-42°C) for 5 min

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YEAST LAB

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  1. YEAST LAB DEAD YEAST W/MINERAL OIL • 2 mL of sucrose solution to 50 mL beaker • Add 2 mL of DEAD yeast; pipette up and down 2-3x to mix • Cover this solution with mineral oil • Incubate beaker in warm water bath (38°-42°C) for 5 min • Place both beakers into biochamber, insert probe and press collect (data should collect every 15 seconds for 4 minutes) • After data collection, restore warm water bath temperature • Fan air over bio-chamber to restore gas levels

  2. YEAST LAB DEAD YEAST W/O MINERAL OIL • Pipette the yeast solution from under mineral oil to clean 50 mL beaker (better to transfer less yeast than to transfer mineral oil) • Incubate beaker in warm water bath (38°-42°C) for 5 min • Place both beakers into biochamber, insert probe and press collect • When asked, choose ‘store latest run’ • Data should collect every 15 seconds for 4 minutes

  3. YEAST LAB LIVE YEAST W/MINERAL OIL • 2 mL of sucrose solution to 50 mL beaker • Add 2 mL of LIVE yeast; pipette up and down 2-3x to mix *Be careful, pipetting too vigorously will kill the yeast • Cover this solution with mineral oil • Incubate beaker in warm water bath (38°-42°C) for 5 min • Place both beakers into biochamber, insert probe and press collect, click ‘store latest run’ (data should collect every 15 seconds for 4 minutes) • After data collection, restore warm water bath temperature • Fan air over bio-chamber to restore gas levels

  4. YEAST LAB LIVE YEAST W/O MINERAL OIL • Pipette the yeast solution from under mineral oil to clean 50 mL beaker (better to transfer less yeast than to transfer mineral oil). • Incubate beaker in warm water bath (38°-42°C) for 5 min • Place both beakers into biochamber, insert probe and press collect (data should collect every 15 seconds for 4 minutes) • When asked, choose ‘store latest run’

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