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Bacterial Transformation Teacher Preparation Sterile Materials

This guide provides step-by-step instructions for preparing sterile materials such as tubes, water, CaCl2 solution, and ampicillin solution essential for bacterial transformation experiments. Ensure proper sterilization for successful results.

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Bacterial Transformation Teacher Preparation Sterile Materials

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  1. Bacterial TransformationTeacher Preparation Sterile Materials

  2. Sterile MaterialsPaper Clips, Glass Pipettes and Toothpicks • Heat the foil packages containing paper clips, glass pipettes, and toothpicks at 350ºF for 30 minutes to sterilize. Bacterial Transformation Teacher Preparation

  3. Sterile Materials1.5 ml Tubes • Sterilize 6- 1.5 ml microcentrifuge tubes for each group of students. • Wrap the tubes in aluminum foil and heat at 250º F for 30 minutes. Bacterial Transformation Teacher Preparation

  4. Sterile MaterialsDistilled Water • In a bottle with a cap, add 50 ml of distilled water. • Cap the bottle and place it in boiling water for 30 minutes. • Refrigerate to prevent contamination. Bacterial Transformation Teacher Preparation

  5. Sterile Materials CaCl2 SolutionStep 1 • Dissolve 0.75 g of CaCl2 into 50 ml of distilled water in a labeled 100-ml glass bottle with a cap. Bacterial Transformation Teacher Preparation

  6. Sterile Materials CaCl2 SolutionStep 2 • Cap the bottle and place it in boiling water for 30 minutes. • Refrigerate to prevent contamination. Bacterial Transformation Teacher Preparation

  7. Sterile MaterialsAmpicillin Solution • Add 1 ml of sterile distilled water to a 50 mg tube of ampicillin powder (provided to Iowa teachers by the Office of Biotechnology). Bacterial Transformation Teacher Preparation

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