1 / 46

Structure et Fonction des Protéines 2

Structure et Fonction des Protéines 2. 3 crédits 24 heures de cours Amphi 2 12 janvier-> 4 mars 8 heures de TP Bio Cell : 8 / 26 mars Bioch : 29 mars 23 avril Responsable Jean-Pierre Duneau jean-pierre.duneau@univmed.fr. Structure et Fonction des Protéines 2. 3 crédits

dominy
Download Presentation

Structure et Fonction des Protéines 2

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Structure et Fonction des Protéines 2 • 3 crédits • 24 heures de cours • Amphi 2 • 12 janvier-> 4 mars • 8 heures de TP • Bio Cell : 8 / 26 mars • Bioch : 29 mars 23 avril • Responsable • Jean-Pierre Duneau • jean-pierre.duneau@univmed.fr

  2. Structure et Fonction des Protéines 2 • 3 crédits • 24 heures de cours (12 jan-> 4 mars)‏ • I Modifications post-traductionnelles des protéines • Pr. Assou El-Battari • 14 heures • Les Mardi à 14h00 + jeudi 4/03 à 8h00 • II Relation Structure-Dynamique-fonction • Jean-Pierre Duneau • 10 heures • Les Jeudi à 8h sauf le 28/01 -> Mercredi 27/01

  3. Maturation structurale et fonctionnelle des protéines 1-Régulation de la traduction des protéines. Rappels 2- Modifications co- et post-traductionnelles La glycosylation 2.1: Les glucides (Rappel) 2.2: La N-glycosylation (modification co-traductionnelle) 2.3: La O-glycosylation (modification post-traductionnelle) 3- Formation des protéoglycanes et glycosaminoglycanes Synthèse de l'héparine et de l'acide hyaluronique 4- Anchrage GPI des protéines membranaires (Glypiation) 5- Autres modifications co- et post-traductionnelles 5.1: Formation des ponts disulfures 5.2: Clivage protéolytique. Exemple des facteurs de coagulation 5.3: Amidation, acylation, sulfatation, phosphorylation

  4. 1- Régulation de la traduction des protéines. Rappels

  5. PP-Dol UDP-GlcNAc Dolicholphosphate N-acétylglucosamine phosphotransférase UMP GlcNAca-PP-Dol UDP-GlcNAc N-acétylglucosaminyl diphosphodolichol N-acétylglucosaminyltransférase UDP GlcNAcb1- 4GlcNAca-PP-Dol CYTOPLASME GDP-Man Dolichol diphosphochitobiose mannosyltransférase GDP Manb1 - 4GlcNAcb1- 4GlcNAca-PP-Dol 4(GDP-Man) Glycolipide a-mannosyltransférases 4GDP Mana1 Mana1 - 2Mana1 6 Manb1 - 4GlcNAcb1 - 4GlcNAca-PP-Dol 3 Mana1 – 2Mana1 Dol-P-Man glycolipide a-mannosyltransférase Flippase Mana1 - 2Mana1 6 Mana1 3 Glycolipide a-glucosyltransférase RETICULUM 6 Manb1 - 4GlcNAcb1 - 4GlcNAca-PP-Dol 3 Mana1 - 2Mana1 Glca1 - 2Glca1 - 3Glca1 - 3Mana1 - 2Mana1 - 2Mana1

  6. P-Dol UDP-GlcNAc Dolicholphosphate N-acétylglucosamine phosphotransférase UMP GlcNAca-PP-Dol UDP-GlcNAc N-acétylglucosaminyl diphosphodolichol N-acétylglucosaminyltransférase UDP GlcNAcb1- 4GlcNAca-PP-Dol GDP-Man Dolichol diphosphochitobiose mannosyltransférase GDP cytoplasme Manb1 - 4GlcNAcb1- 4GlcNAca-PP-Dol 4(GDP-Man) Glycolipide a-mannosyltransférases 4GDP RE Mana1 6 Manb1 - 4GlcNAcb1 - 4GlcNAca-PP-Dol 3 lumière Mana1 - 2Mana1 - 2Mana1 4(Manb-P-Dol) Dol-P-Man glycolipide a-mannosyltransférase Mana1 – 2Mana1 4Dol-P 6 Mana1 3 6 Manb1 - 4GlcNAcb1 - 4GlcNAca-PP-Dol 3 Mana1 - 2Mana1 Mana1 - 2Mana1 - 2Mana1 3(Glcb-P-Dol) Glycolipide a-glucosyltransférase 3Dol-P Mana1 - 2Mana1 6 Mana1 3 6 Manb1 - 4GlcNAcb1 - 4GlcNAca-PP-Dol 3 Mana1 - 2Mana1 Glca1 - 2Glca1 - 3Glca1 - 3Mana1 - 2Mana1 - 2Mana1 Peptide-(Asn-X-Ser/Thr) oligosaccharidyltransférase Mana1 - 2Mana1 PP-Dol 6 Mana1 3 6 Manb1 - 4GlcNAcb1 - 4GlcNAcb-Asn.peptide 3 Mana1 - 2Mana1 Glca1 - 2Glca1 - 3Glca1 - 3Mana1 - 2Mana1 - 2Mana1 R Mannosyl-oligosaccharide a-glucosidase I 1 Glc a-glucosidase II 1 Glc UDP-Glc:a1,3glucosyltransférase Mana1 - 2Mana1 Mana1 - 2Mana1 UDP UDP-Glc 6 Mana1 3 6 Mana1 3 6 Manb1 - R 3 6 Manb1 - R 3 Mana1 - 2Mana1 Mana1 - 2Mana1 Glc Glca1 - 3Mana1 - 2Mana1 -2Mana1 Mana1 - 2Mana1 - 2Mana1 a-glucosidase II Mana1 - 2Mana1 Mannosyl-oligosaccharide 1,2-a-mannosidase IA 1 Man 6 Mana1 3 6 Manb1 - R 3 Mana1 Mana1 - 2Mana1 - 2Mana1 Figure 1 : Biosynthèse des N-glycannes dans le RE

  7. Golgi ERp57 (disulphide isomerase) protein folding on/off calnexin cx G II P a-glucosidase II ERGIC-53 EDEM P glucosyl- transferase mannosidase P other chaperones ER Glc EDEM P Glc a-glucosidase I & II Glc Man Man P Sec61 proteasome

  8. GnT V GlcNAcb1 6 4 Mana1 3 GnT VI GlcNAcb1 GlcNAcb1 GnT II 6 4Manb1 - 4GlcNAcb1 - 4GlcNAcb1 - Asn 3 GlcNAcb1 GnT III GlcNAcb1 GnT IV 6 4 Mana1 3 GnT I GlcNAcb1

  9. Mana1 6 Mana1 3 Mana1 6 Manb1 - R 3 6 Manb1  R 3 Mana1 Mana1 Mana1 Mana1 6 Mana1 3 Mana1 6 Mana1 3 Fuca1 Fuca1 6 GlcNAcb1- 4Manb1 - R,R ’ 3 Mana1 6 Manb1 - R 3 6 4GlcNAcb1- 4GlcNAcb-Asn-peptide. 6 4GlcNAcb1- 4GlcNAcb-Asn-peptide Mana1 GlcNAcb1-2Mana1 GlcNAcb1-2Mana1 Mana1 Mana1 6 GlcNAcb1- 4Manb1 - R,R ’ 3 6 Manb1 - R,R ’ 3 Mana1 6 Manb1 - R,R ’ 3 GlcNAcb1-2Mana1 GlcNAcb1-2Mana1 GlcNAcb1 4 Mana1 2 GlcNAcb1 GlcNAcb1-2Mana1 GlcNAcb1-2Mana1 6 GlcNAcb1- 4Manb1 - R,R ’ 3 6 Manb1 - R, R ’ 3 GlcNAcb1-2Mana1 GlcNAcb1-2Mana1 GlcNAcb1 GlcNAcb1 6 Mana1 2 6 Mana1 2 GlcNAcb1-2Mana1 GlcNAcb1- 2Mana1 GlcNAcb1 6 GlcNAcb1- 4Manb1 - R,R ’ 3 6 Manb1  R,R ’ 3 6 GlcNAcb1- 4Manb1 - R,R ’ 3 6 Manb1 - R,R ’ 3 GlcNAcb1 GlcNAcb1 GlcNAcb1 4 Mana1 2 4 Mana1 2 GlcNAcb1-2Mana1 GlcNAcb1-2Mana1 GlcNAcb1 GlcNAcb1 GlcNAcb1 GlcNAcb1 6 GlcNAcb1 - 4Mana1 2 GlcNAcb1 6 Mana1 2 GlcNAcb1 6 Mana1 2 6 GlcNAcb1- 4Mana1 2 6 GlcNAcb1 - 4Manb1 - R,R ’ 3 GlcNAcb1 6 GlcNAcb1- 4Manb1 - R,R ’ 3 GlcNAcb1 6 Manb1 - R,R ’ 3 GlcNAcb1 6 Manb1 - R,R ’ 3 GlcNAcb1 GlcNAcb1 GlcNAcb1 GlcNAcb1 4 Mana1 2 GlcNAcb1 4 Mana1 2 4 Mana1 2 4 Mana1 2 GlcNAcb1 GlcNAcb1 GlcNAcb1 GlcNAcb1 Mana1 - 2Mana1 6 Mana1 3 6 Manb1 - R 3 Mana1 Mana1 - 2Mana1 - 2Mana1 Mannosyl-oligosaccharide1,3-1,6-a-mannosidase III Mannosyl-oligosaccharide 1,2-a-mannosidase IB GnT I Glycoprotéine-6-a-L-fucosyltransférase GnT III R ’ Mannosyl-oligosaccharide1,3-1,6-a-mannosidase II GnT III GnT I GnT IV GnT II GnT III GnT IV GnT V GnT III GnT III GnT IV GnT V GnT III GnT III GnT VI Figure 4 : Action des GnTs dans la biosynthèse des N-glycannes. R= 4GlcNAcb1 -4GlcNAcb-Asn.peptide, R ’=

More Related