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Computer Simulation of Neurophysiology

Computer Simulation of Neurophysiology. Presented in Lab. Computer Simulation of Neurophysiology. Action Potential Stimulation Determine threshold Observe effects of other stimuli Effects of Drugs Determination of Conduction Velocity. Action Potential Generation: Stimulus.

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Computer Simulation of Neurophysiology

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  1. Computer Simulation of Neurophysiology Presented in Lab

  2. Computer Simulation of Neurophysiology • Action Potential Stimulation • Determine threshold • Observe effects of other stimuli • Effects of Drugs • Determination of Conduction Velocity

  3. Action Potential Generation:Stimulus • Action potentials are triggered by membrane depolarization at the axon hillock • Depolarization caused by increased permeability to ions • Permeability typically increased by chemically-or physically-gated ion channels • Can also be affected by membrane perturbations, changes in ion gradients, etc.

  4. Neuron Axon Response Threshold Stimulus Intensity Threshold and “All or None” • Action potentials are driven by the opening of voltage-gated ion channels • Require a minimum amount of depolarization for any to open = threshold • Once some open, they in turn cause others to open • “All or none” response for single neurons

  5. Compound Action Potentials • Extracellular recordings of whole nerve segments • Many axons w/ variable thresholds • Different degrees of stimulation • Amplitude of Action Potential varies with stimulus strength • Vary NUMBER of axons undergoing AP • Does NOT violate “all or none” principle Stimulus Intensity Action Potential Amplitude

  6. Compound Action Potentials maximal • Subthreshold stimulus • No AP • Threshold stimulus • Minimal compound AP • Submaximal stimulus • Variable number of axons undergo AP • Variable amplitude • Maximal stimulus • Maximum amplitude • Does not vary with stim strength submaximal threshold subtheshold Stimulus Intensity Action Potential Amplitude

  7. Nerve signaling and drugs • Signal conduction within a neuron occurs through action potentials • voltage-gated channels • Signal conduction from one nerve to another occurs through synapses • Chemically gated channels • Neurotoxins could affect nerve signaling at either site Lecture Text Fig 7.22

  8. Neurotoxins • Affect voltage-gated ion channels (alter APs) • Tetradotoxin, novocain – block v.g. Na+channels • Scorpion venoms – keep Na+ channels open and K+ channels closed (prolonged depolarization) • Chlorform – open K+channels (hyperpolarization)

  9. Neurotoxins • Affect chemistry at synapses (alter normal AP-inducing stimuli) • Botulinum toxin (Botox) – prevents vesicle release from somatic motor neurons • Latrotoxin (Black widows) – triggers excessive vesicle release from somatic motor neurons • Cobratoxin (Cobras) – blocks nicotinic receptors

  10. Factors Affecting Signal Conduction: Myelination • myelin = lipid insulator • PM of Schwann cells or oligodendrocytes • Signals “jump” from one node to the next (saltatory conduction) • AP conduction speed Lecture Text Fig 7.18 http://www.blackwellscience.com/matthews/actionp.html

  11. Factors Affecting Signal Conduction: Axon Diameter • Cable Theory • resistance to current increases with decreased diameter • resistance slows current • Therefore: • Conduction Velocity a 1/Resistance • Diameter a 1/Resistance • Conduction velocity a Diameter

  12. Neurophysiology Background Material (Not Presented in Lab)

  13. Resting Potential • Inside of cell negative relative to the outside (-70 mV) • [Na+] higher outside than inside • [K+] higher inside than outside • At RP, neither K+ nor Na+ are in equilibrium Fig 3A

  14. Action Potentials • begins at the axon hillock, travels down axon • brief, rapid reversal of MP • Opening of voltage-gated Na+ and K+ channels • Self propagating • All or none Lecture Text Fig 7.17

  15. Action Potential Function(Depolarization) • Triggering event causes membrane to depolarize • slow increase until threshold is reached • voltage-gated Na+ channels open • Na+ enters cell • further depolarization • more channels open • further depolarization • membrane reverses polarity (+30 mV) Figs 3B, 3C

  16. Action Potential Function(Repolarization) • V.G. Na+ channels close • Delayed opening of V.G. K+ channels • reach peak permeability as Na+ channels close • K+ rushes out of the cell • membrane potential restored • K+ channels close • [Na+] and [K+] restored by the Na+-K+ pump Figs 3C, 3D

  17. Cartoons! http://www.blackwellscience.com/matthews/channel.html

  18. Action Potentials • AP duration ~ 1-2 ms • response of the nerve cell to the stimulus is “all or none” • Amt of depolarization always the same • differences in stimulus intensity are detected by • The number of neurons undergoing AP in response to the stimulus • The frequency of action potential generation Lecture Text Fig 7.15

  19. Action Potential Propagation • Na+ moving into one segment of the neuron quickly moves laterally inside the cell • Depolarizes adjacent segment to threshold Lecture Text Fig 7.17 http://www.blackwellscience.com/matthews/actionp.html

  20. Chemical Synapses • presynaptic neuron • synaptic terminal bouton • contains synaptic vesicles filled with neurotransmitter • synaptic cleft • space in-between cells • postsynaptic neuron • Subsynaptic membrane • Receptor proteins for neurotransmitter Lecture Text Fig 7.21

  21. Chemical Synapses • AP in terminal opens Ca2+ channels • Ca2+ rushes in. • Ca2+ causes vesicles to fuse to PM and release contents • Transmitter diffuses across synaptic cleft and binds to receptors on subsynaptic membrane Lecture Text Fig 7.21

  22. Chemical Synapses • Specific ion channels in subsynaptic membrane open • chemically-gated ion channels • Ions enter postsynaptic cell • graded potential forms • If graded potential is strong enough to reach threshold, generates action potential in postsynaptic cell Lecture Text Figs 7.23, 7.24

  23. Cartoon! http://www.blackwellscience.com/matthews/nmj.html

  24. Types of Chemical Synapse • Excitatory chemical synapse: • excitatory postsynaptic potentials (EPSPs) • Small depolarization of postsynaptic neuron • closer to threshold Lecture Text Fig 7.26 http://www.blackwellscience.com/matthews/neurotrans.html

  25. Types of Chemical Synapse • Inhibitory chemical synapse: • inhibitory postsynaptic potentials (IPSPs) • Small hyperpolarization of postsynaptic neuron • further from threshold Lecture Text Fig 7.30 http://www.blackwellscience.com/matthews/neurotrans.html

  26. Synaptic Integration • Multiple synaptic events have an additive effect on membrane potential • summation • Sum of inputs determines whether axon hillock depolarized enough for AP to form.

  27. Spatial Summation • numerous presynaptic fibers may converge on a single postsynaptic neuron • additive effects of numerous neurons inducing EPSPs and IPSPs on the postsyn. neuron Lecture Text Figs 7.29, 7.30

  28. Temporal Summation threshold • additive effects of EPSPs and IPSPs occurring in rapid succession • next synaptic event occurs before membrane recovers from previous event membrane potential stimulus Low Frequency Stimulation threshold membrane potential stimulus High Frequency Stimulation

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