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Peptide + no CpG Low dose peptide + CpG Plasmid. CD8 + PD-1 high. CD8 + PD-1 low. CD8 + PD-1 low. Vaccination. Antigen stimulation + anti-PD-1 Ab. Ex vivo CFSE staining of T cells. FACS analysis (proliferation).
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Peptide + no CpG Low dose peptide + CpG Plasmid CD8+ PD-1high CD8+ PD-1low CD8+ PD-1low Vaccination Antigen stimulation + anti-PD-1 Ab Ex vivo CFSE staining of T cells FACS analysis (proliferation)