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1 kb SUPPLEMENTARY MATERIAL S 1. Fusion of lacZ to actP (acetate permease) to give plasmid pRB71. The fusion sequence comprises 450 bp upstream of the first ORF of the operon, the entire acs (acetyl-CoA synthetase) and the first 317 bp of actP (See plasmids construction for further details). hypothetical protein hypothetical protein acs actP pRB71 P lacZ P