A convenient system for highly specific and sensitive detection of miRNA expression

1. SUPPLEMENTAL DATA A convenient system for highly specific and sensitive detection of miRNA expression Xiangqi Li, Minjie Ni, Chaobao Zhang, Wubin Ma, Yonglian Zhang

2. Fig. S1 Detection of miRNAs under different conditions by stem-loop PCR. miR-125a-3p Marker miR-122 miR-291 miR-326 NO miR-138 miR-99b＊ miR-292-5p miR-295 miR-3085 A 100bp 50bp miR-125a-3p Marker miR-122 miR-291 miR-326 NO miR-138 miR-99b＊ miR-292-5p miR-295 miR-3085 B 100bp 50bp miR-125a-3p Marker miR-122 miR-291 miR-326 NO miR-138 miR-99b＊ miR-292-5p miR-295 miR-3085 C 100bp 50bp

3. Fig. S2 The sensitivity of the detection of miR-7578 and distinguishing miR-7578 from its precursor by northern blotting. 1 zmol 1 amol 1 fmol 10 fmol Pre-miR-7578 Rno-miR-7578 Fig. S3 Detection of miR-29a in different cell lines by different methods. JEG-3 Cell U-2 OS cell 5637 Cell MG-63 Cell T24 Cell A Rno-miR-29a by northern blotting B Rno-miR-29a by aLHCD

4. Fig. S4 Quantitative detection of miRNA levels using U6 as an internal control by LH-PCR.

5. Fig. S5 Evaluation of LH-PCR amplification efficiencies of miRNA and its precursor.

6. Fig. S6 Evaluation of LH-PCR amplification efficiencies of miRNA and its family members.

7. Fig. S7 Detection of miR-29 family members by conventional Northern blotting. Tissue RNA miR-29b miR-29c miR-29a miR-29a Fig. S8 Detection of miR-138 and its precursor from tissue samples by stem loop-PCR . pre Hela liver kidey lung brain Rno-miR-138