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Photosynthesis!!!!. Photosynthesis is divided into two parts. The overall reaction in photosynthesis:. Light energy. 6CO 2. 12 H 2 O. C 6 H 12 O 6. 6O 2. 6 H 2 O. +. +. +. +. Two components:. Light-dependent reactions. Light-independent reactions. Chemical energy
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Photosynthesis is divided into two parts The overall reaction in photosynthesis: Light energy 6CO2 12 H2O C6H12O6 6O2 6 H2O + + + + Two components: Light-dependent reactions Light-independent reactions Chemical energy (ATP, NADPH) Chemical energy (ATP, NADPH) Chemical energy (C6 H12O6) Light energy O2 H2O CO2 Energy Harvest Synthesis
Figure 10.4 An overview of photosynthesis: cooperation of the light reactions and the Calvin cycle (Layer 3)
Photosynthesis starts in the thylakoid membrane These aggregations of pigment molecules are called photosystems
The energy from light is captured and converted in chloroplasts • Energy from light excites an electron in chlorophyll. This electron is then passed to acceptor molecules to be used to make ATP and NADPH Stroma Light photon Electron acceptor molecule e- Reaction center (chlorophyll a ) Thylakoid Thylakoid space Chloroplast Photosystem Stroma
There are 2 types of reaction centers in green algae and plants: Photosystem I: activated by light of wavelengths of 700nm Photosystem II: activated by light of wavelengths of 680nm These photosystems are named after the order in which they were discovered, not the order in which they act during photosynthesis
Noncyclic electron flow during the light reactions generates ATP and NADPH
The electrons in photosystem II are replaced by electrons from water
The electrons from chlorophyll are passed to an ETC which makes ATP
The electrons reach photosystem I where another photon of light excites them once again
The electrons from photosystem II are passed down a second ETC, in which they are used to make NADPH
Figure 10.16 The light reactions and chemiosmosis: the organization of the thylakoid membrane
Figure 10.15 Comparison of chemiosmosis in mitochondria and chloroplasts
Sometimes an alternate pathway happens In cyclic electron transport, which drives cyclic photophosphorylation, photosystem I transfers electrons to plastoquinone (PQ).
Summary of the Light Dependent Reaction of Photosynthesis • Light energy is absorbed by chlorophyll molecules, which excites electrons • The energy from the excited electrons is converted to chemical energy: • Photosystem II: • electron transport chain produces ATP • The excited electrons are replaced by splitting a water molecule, releasing O2 • Photosystem I: • The excited electron is donated to NADP+ to produce NADPH
Photosynthesis is a two-step process • The light independent reactions (Calvin cycle) use the energy in ATP and NADPH to reduce organic compounds to form glucose.
Summary • Light Dependent Reaction • Light + chlorophyll --> ATP + NADPH + (O2 as waste) • Light Independent Reaction (Calvin Cycle) • CO2 + ATP + NADPH --> glucose
What happens to the glucose produced by photosynthesis? Sucrose CH2OH O O HOCH2 H H H H HO OH H O H CH2OH HO H OH HO H Fructose subunit Glucose subunit
Starch CH2OH CH2OH CH2OH O O O H H H H H H Up to 1000or moremonomers H H H OH OH H H H OH O O O O OH H OH H OH H Glucosesubunit Glucosesubunit Glucosesubunit