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Ion Channels as Natural NanoDevices 1

This project aims to develop tools to control ion channels for practical use by measuring currents from protein molecules, fabricating channel sheets, and understanding selectivity. Biomimetics of ion channels into nanopores for single molecule detection.

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Ion Channels as Natural NanoDevices 1

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  1. Porins Gramicidin Ion Channels as Natural NanoDevices1 • Project Goals: Develop measurement, simulation, and fabrication tools needed to make ion channels into practical devices that can be controlled for our use. • Technical Approach • Measure currents from single protein molecules of known and modified ATOMIC structure, in a wide range of solutions • Compare measurements with currents predicted from mean field theories and transport Monte Carlo, so we understand the PHYSICAL BASIS OF SELECTIVITY and permeation. • Fabricate sheets of channels as strong as SaranWrap that generate microamps of signal. Channel Sheets might be the silicon wafer of ionic transistors.

  2. Calcium Channel Na Channel Cl Channel AgonistChannel Ion Channels as Natural NanoDevices2 Technical Approach • Compare measurements with currents predicted from mean field theories and transport Monte Carlo, so we understand the PHYSICAL BASIS OF SELECTIVITY and Permeation.

  3. Ion Channels, Nanopore Sensors, Aluru et al 3 Figure from Mashl/Jakobsson • Biomimetics: Functionality of ion channels into nanopores (CNTs) • Ideal for IC based chips • Fundamental issues: Transport of water, electrolytes and analytes through CNT • Single molecule detection • Power of protein engineering • Current flow changed by binding • Frequency reveals concentration • Amplitude reveals identity • Durable only in lab setting

  4. Occupancy of Water in SWCNT 4 Our Simulations Nature 414, 188 - 190 (2001)

  5. Drift-Diffusion Simulation with Prophet5Umberto Ravaioli et al • It is straightforward to include several multi-valent species in PROPHET without modifying the source-code. Example: CaCl2 in ompF porin

  6. Monte Carlo Particle Simulation of Channels 6 Umberto Ravaioli et al The charged particle trajectories are evaluated froma detailed electric field distribution in space, obtained by solving the Poisson equation. Gramicidin - Successful Na+ trajectory – Point particle model

  7. Ion Channels as Natural NanoDevices7 Technical Approach • Fabricate sheets of channels as strong as SaranWrap that generate microamps of signal. Channel Sheets might be the silicon wafer of ionic transistors.

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