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culturing bioluminescent bacteria

culturing bioluminescent bacteria. dead fish, i love you, but you’re bringing me down. b acterial bioluminescence. Is interesting Diverse Vibrios in the sea Goal: to isolate bioluminescent bacteria for further study. strategy.

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culturing bioluminescent bacteria

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  1. culturing bioluminescent bacteria dead fish, i love you, but you’re bringing me down

  2. bacterial bioluminescence • Is interesting • Diverse Vibriosin the sea • Goal: to isolate bioluminescent bacteria for further study

  3. strategy • Bioluminescent bacteria are present throughout marine environments • Bioluminescence is detectable when cells are present at high density • Therefore: make bacteria grow to induce luminescence, pick them out

  4. Seawater complete media • Artificial seawater, bactotryptone, yeast extract, glycerol, agar

  5. Photo courtesy S. Russell

  6. How do we improve yields? • Sample from enriched locations • Improve culturability (great plate count anomaly) • In situ culturing with diffusion chambers? D’Onofrio et al (2010) Chemistry & Biology. 17(3):256-264

  7. How to improve yields? (cont’d) • More selective media, or bait? • chemotaxis • Non-symbiosis-related functions of bioluminescence? • Improve detection on plate

  8. the end

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