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The Effects of Hydrocarbons on Bioluminescent Bacteria

The Effects of Hydrocarbons on Bioluminescent Bacteria. By: Tobi Simon. Need. Long-term exposure Plants and Animals. http://www.peternjenga.com/blogs/greenkenya/wp-content/uploads/2009/03/natural-and-manufactured-air-pollution.png. Knowledge Base.

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The Effects of Hydrocarbons on Bioluminescent Bacteria

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  1. The Effects of Hydrocarbons on Bioluminescent Bacteria By: Tobi Simon

  2. Need Long-term exposure Plants and Animals http://www.peternjenga.com/blogs/greenkenya/wp-content/uploads/2009/03/natural-and-manufactured-air-pollution.png

  3. Knowledge Base http://nptel.iitm.ac.in/courses/Webcourse-contents/IIT-Delhi/Environmental%20Air%20Pollution/air%20pollution%20(Civil)/Module-1/images1/35.gif

  4. Knowledge Base Bioluminescent Bacteria http://www.mcbs.unh.edu/faculty/Whistler/images/image3.jpg

  5. Literature Review Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of Environmental Quality (2006).

  6. Literature Review • Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Samples." Journal of Environmental Quality (2008).

  7. Literature Review • Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988).

  8. Literature Review • Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal of Environmental Quality (2005).

  9. Purpose The effects of hydrocarbons on the amount of light emitted from bioluminescent bacteria.

  10. Hypothesis Alternate: Water with higher amounts of hydrocarbons will cause a decrease in the amount of light emitted from the bacteria Null: Water with hydrocarbons in it will have no effect on the amount of light emitted from the bacteria.

  11. Methodology Water is collected from different locations and a sample of each is tested for the amount of hydrocarbons present using a spectrophotometer Different samples of water are placed into the cultures of bioluminescent bacteria Control No hydrocarbon in water (tap water) Variable 1 Lowest amount of hydrocarbon in water Variable 2 Medium amount of hydrocarbon in water Variable 3 Highest amount of hydrocarbon in water After 24 hours the BLR will be calculated for each sample of water. Statistical Analysis: ANOVA followed by a Sheffe post hoc test

  12. Do Ability Organisms Procedures http://www.abdn.ac.uk/staffpages/uploads/bch052/aglover-bac.jpg

  13. Budget

  14. Bibliography • American College of Gastroenterology. “Air Pollution May Increase Risk Of Appendicitis.” Science Daily 7 October 2008. 23 April 2009 • <http://www.sciencedaily.com- /releases/2008/10/081006102537.htm> • Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Samples." Journal of Environmental Quality (2008). • Bernstein, Leonard. Environmental Science Ecology and Human Impact. 2nd ed. Menlo Park, California: Addison-Wesley Company, 1996. • "Bioluminescence." McGraw-Hill Encyclopedia of Science and Technology. 10th ed. 2007. • Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of Environmental Quality (2006). • Bullock, Carole. “Air pollution may cause and speed up artery disease.” EurekAlert! • Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal of Environmental Quality (2005). • Gulifoy, Christine. “Inhaling a heart attack: How air pollution can cause heart disease.” EurekAlert! • International Union of Forest Research Organizations. “Damage To Forests From Climate Change Could Cost The Planet Its Major Keeper Of Greenhouse Gases, Study Warnes.” ScienceDaily. 21 April 2009. • Safra, Jacob E. "Bioluminescence." The New Encyclopedia Britannica. 15th ed. • University of Arizona. “Fire Influences Global Warming More Than Previously Thought.” ScienceDaily 29 April 2009. • http://www.sciencedaily.com/releases/2009/04/090423142332.htm. • University of Oxford. “Ice Sheet Behavior Much More Volatile And Dynamic Than Previously Thought, Tahiti Corals Show.” ScienceDaily 30 April 2009. 29 April 2009 • <http://www.sciencedaily.com/releases/2009/04/090423205104.htm> • Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988). •  http://www.lungusa.org/atf/cf/%7B7A8D42C2-FCCA-4604-8ADE-7F5D5E762256%7D/key_air.pdf

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