0.05

1. Riesia pediculicola, P-symbiont of human body louse Pediculus humanus [AB263101] AT 50.6% 0.05 99/88/100 Riesia pediculicola, P-symbiont of human head louse Pediculus capitis [AB263105] AT 51.0% 99/100/100 Riesia pediculischaeffi, P-symbiont of chimpanzee louse Pediculus schaeffi [EF110573] AT 50.3% 94/100/96 Riesia phthiripubis, P-symbiont of human pubic louse Pthirus pubis [EF110574] AT 52.4% */100/* Arsenophonus nasoniae, S-symbiont of parasitoid wasp Nasonia vitripennis [AY264674] AT 45.9% */98/* Arsenophonus arthropodicus, S-symbiont of louse fly Pseudolynchia canariensis [DQ115536] AT 46.0% 95/96/100 Hamiltonella defensa, S-symbiont of aphid Acyrthosiphon pisum [AF293616] AT 45.9% Regiella insecticola, S-symbiont of aphid Acyrthosiphon pisum [AF293618] AT 45.7% 99/100/100 Serratia symbiotica, S-symbiont of aphid Acyrthosiphon pisum [M27040] AT 45.9% Escherichia coli [J01695] AT 45.6% Serratia marcescens [AF124042] AT 45.5% Baumannia cicadellinicola, P-symbiont of sharpshooter Homalodisca coagulata [AY676877] AT 51.6% P-symbiont of macaque louse Pedicinus obtusus (Jigokudani) [AB478978] AT 53.5% 100/100/100 P-symbiont of macaque louse Pedicinus obtusus (Yakushima) [AB478979] AT 53.5% 72/94/* Wigglesworthia glossinidia, P-symbiont of tsetse fly Glossina brevipalpis [NC_004344] AT 51.2% Symbiont of bovine louse Haematopinus eurysternus [DQ076661] AT 52.2% 97/100/100 Symbiont of pig louse Haematopinus suis [DQ076662] AT 51.1% 100/100/100 Symbiont of bore louse Haematopinus apri [DQ076665] AT 51.9% S-symbiont of psyllid Calophya schini [AF263560] AT 54.5% */*/80 Symbiont of elephant louse Haematomyzus elephantis [DQ076663] AT 55.3% Blochmannia floridanus, P-symbiont of ant Camponotus floridanus [AY334381] AT 52.2% Ishikawaella capsulata, P-symbiont of plataspid stinkbug Megacopta punctatissima [AB240157] AT 50.7% Buchnera aphidicola, P-symbiont of aphid Acyrthosiphon pisum [M27039] AT 50.0% 96/81/* 84/*/95 Buchnera aphidicola, P-symbiont of aphid Yamatocallis tokyoensis [AB064507] AT 52.1% 100/100/100 Buchnera aphidicola, P-symbiont of aphid Geoica urticularia [AJ296751] AT 51.2% P-symbiont of grain weevil Sitophilus oryzae [AF005235] AT 45.7% FIG. 1. Molecular phylogenetic placement of the symbiont of P. obtusus on the basis of 16S rRNA gene sequences. A maximum- likelihood (ML) tree inferred from 593 unambiguously-aligned nucleotide sites is shown; Bayesian (BA) and neighbor-joining (NJ) analyses gave essentially the same results. Statistical support values higher than 70% are indicated at the nodes in the order of ML/BA/NJ. Asterisks indicate statistical support values lower than 70%. Sequence accession numbers are shown in brackets. AT contents of the sequences are also shown. The sequences from the symbionts of the primate, animal and bird lice are highlighted in boldface. Sodalis glossinidius, S-symbiont of tsetse fly Glossina morsitans [AY861701] AT 45.5% P-symbiont of pigeon louse Columbicola columbae [AB303382] AT 45.7% 100/ 100/ 100 Symbiont of louse fly Craterina melbae [EF174495] AT 44.4% Vibrio cholerae [X74694] AT 46.3%