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4. 0.25. A. B. 100. wild type membrane. wild type membrane. 3. wild type soluble. wild type soluble. 0.2. 75. SfrAB-null membrane. 2. 0.15. SfrAB-null soluble. %Total Activity. Specific activity ( µ molmg -1 min -1 ). 50. 0.1. 1. 25. 0.05. 0. 0. 0. reductase

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  1. 4 0.25 A B 100 wild type membrane wild type membrane 3 wild type soluble wild type soluble 0.2 75 SfrAB-null membrane 2 0.15 SfrAB-null soluble %Total Activity Specific activity (µmolmg-1min-1) 50 0.1 1 25 0.05 0 0 0 reductase activity: NADPH/ Fe(III)-NTA NADPH/ AQDS NADPH/ viologen NADPH/ Fe(III)-NTA NADPH/ AQDS NADPH/ viologen oxoglutarate/ viologen Fig. S2. Preliminary biochemical characterization of an independently isolated, acetate-adapted SfrAB-null strain. (A) Determination of NADPH-dependent Fe(III)-NTA, AQDS, and benzyl viologen reductase activities in membrane and soluble fractions prepared from the wild-type and SfrAB-null strains. (B) Distribution of the cytoplasmic enzyme, oxoglutarate/ferredoxin oxidoreductase, and the various reductase activities in the membrane and soluble fractions of the wild-type strain. Coppi et al.(2007) Microbiology153, 3572-3585

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