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Choosing the Appropriate Camera

Choosing the Appropriate Camera. “Resolving” the Application. The Appropriate Camera. If you know the application AND the product, you will win every time If you know either the application or the product, you may win half the time If don’t know either, you will lose.

ethan-bernard
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Choosing the Appropriate Camera

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  1. Choosing the Appropriate Camera “Resolving” the Application

  2. The Appropriate Camera • If you know the application AND the product, you will win every time • If you know either the application or the product, you may win half the time • If don’t know either, you will lose

  3. How do I Select the Correct Product? • Guess • Pick The Cheapest • Pick the Most Expensive • Call Scott Monroe • “Resolve” the Application

  4. Resolving the Application: Provide the Appropriate Resolution for the Application

  5. Appropriate “Resolution” Spatial Temporal Intensity

  6. Spatial Resolution Spatial

  7. Spatial Resolution Size of pixels ? 0.61 . Mag = 2 pixels 100 x 1.3 NA N.A.

  8. Spatial Resolution Number of pixels Field of View !!

  9. Temporal Resolution Temporal

  10. Temporal Resolution • What is your “Real Time” • How much light do you have in a given time interval?Considerations: • Digitizer Readout Rate • CCD Architecture • Quantum Efficiency • Read Noise

  11. Temporal Resolution Small Array Size Interline Region of Interest Frame Transfer

  12. Temporal Resolution e e ICCD

  13. Intensity Resolution Intensity

  14. Intensity Resolution • Dim and Bright sources within the same Image • Measure small differences in Intensities • Measure small Fluctuations on a large background Dynamic Range

  15. Intensity Resolution Signal Mapping to the ADC 4095 Gray Values 0 20,000 40,000 80,000 electrons

  16. Best of “Both Worlds” High Spatial and Intensity Resolution Spatial Temporal Intensity

  17. Best of “Both Worlds” High Spatial and Intensity Resolution Full Frame Acquisition

  18. Best of “Both Worlds” High Temporal and Intensity Resolution Spatial Temporal Intensity

  19. Best of “Both Worlds” High Temporal and Intensity Resolution Binning: On Chip Charge Combination

  20. Best of “Both Worlds” High Temporal and Intensity Resolution On-Chip Multiplication Gain

  21. Best of “Both Worlds” High Spatial and Temporal Resolution Spatial Temporal Intensity

  22. ( X , Y ) ( X , t ) Best of “Both Worlds” High Spatial and Temporal Resolution “Shoot and Shift” or “Kinetics Mode”

  23. ( X , Y ) ( x, y , t ) Best of “Both Worlds” High Spatial and Temporal Resolution “Virtual Chip Mode”

  24. Biological Products

  25. Microscopy Products • Tier 1 • CoolSNAPcf Color and Mono • Tier 2 • CoolSNAPES and CoolSNAPHQ • Tier 3 • SenSys, Quantix, Cascade • Tier 4 • MicroMAX Backthinned, I-PentaMAX

  26. Price Points: 8x 4-6x 3x 2x 1x

  27. Biological Products

  28. Roper Scientific Cameras:CoolSNAP HQ/es/cf Spatial Temporal 20MHz Interline Architecture 1.3 Mega Pixels Small Pixels Intensity

  29. Roper Scientific Cameras HQ/es/cf Spatial Temporal 1.4 Mega Pixels Small Pixels SenSys Full Frame Architecture Intensity

  30. Roper Scientific Cameras HQ/es/cf Spatial Temporal Frame Transfer Architecture SenSys 512BFT Intensity 16-bit 100kHz, 1MHz

  31. Roper Scientific Cameras HQ/es/cf Spatial Temporal Frame Transfer Architecture QT 57 SenSys 512BFT Intensity 12-bit, 1-3MHz

  32. Roper Scientific Cameras HQ/es/cf Spatial Temporal 1.0 Mega Pixels QT 57 SenSys 512BFT 1024B Intensity 16-bit 100kHz, 1MHz Large pixels

  33. Roper Scientific Cameras HQ/es/cf Spatial Temporal Gen 3 Intensifier Frame Transfer Architecture I-PMax QT 57 SenSys 512BFT 1024B Intensity

  34. Roper Scientific Cameras HQ/es/cf Spatial Temporal On-Chip Multiplication Gain Frame Transfer Architecture I-PMax QT 57 SenSys 512B 512BFT 1024B Intensity 16 micron pixels

  35. Where Does Your Application Fit? HQ/es/cf Spatial Temporal ? I-PMax QT 57 SenSys 512B 512BFT 1024B Intensity

  36. Where Does Your Application Fit? Cortical Imaging ala Toga HQ/es/cf Spatial Temporal ? I-PMax QT 57 SenSys 512B 512BFT 1024B Intensity

  37. Where Does Your Application Fit? Tirf Res/Tirf Fluor HQ/es/cf Spatial Temporal ? I-PMax QT 57 SenSys 512B 512BFT 1024B Intensity

  38. Where Does Your Application Fit? Embyonic Chick HQ/es/cf Spatial Temporal ? I-PMax QT 57 SenSys 512B 512BFT 1024B Intensity

  39. Where Does Your Application Fit? Single Molecule Fluor HQ/es/cf Spatial Temporal ? I-PMax QT 57 SenSys 512B 512BFT 1024B Intensity

  40. Where Does Your Application Fit? Live Cell Fluor HQ/es/cf Spatial Temporal ? I-PMax QT 57 SenSys 512B 512BFT 1024B Intensity

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