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Oral presentation

Clear Interesting. Oral presentation. Lab meeting. Journal club. Qual. Thesis. Presentation at meeting. Choice of a paper. Goal: 1. On topic 2. Important and interesting to a large audience. Localization AND ( Cell [ta] OR Science [ta] OR Nature [ta]). Nature Methods.

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Oral presentation

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  1. Clear Interesting Oral presentation Lab meeting Journal club Qual Thesis Presentation at meeting

  2. Choice of a paper Goal: 1. On topic 2. Important and interesting to a large audience Localization AND(Cell [ta]OR Science [ta]OR Nature [ta]) Nature Methods

  3. Introduction 2-3 slides From general to particular

  4. Introduction-Example 1

  5. Significance? Question? What do I need to know? Scheme/visuals? Punch line? Introduction-Example 1 • Transcription is important in neural stem cells • SMRT=NcoR2 is a nuclear co-repressor • Histones get methylated at lysine 27. • There are demethylases that target these lysines • SMRT -/- mice have defects in the cortex.

  6. Neural stem cells neural stem cells progenitors neuronal glial Development of the nervous system Regeneration of adult brain

  7. Transcriptional regulation in neurogenesis? SMRT Program of target genes? Neuronal fate

  8. Localization of molecules In situ hybridization of RNA: Target genes for SMRT Immunofluorescence:Role of SMRT on cell fate (neuronal versus glial markers)

  9. Approaches 1-2 slides 1 scheme is better than 1000 words Emphasize controls and limitations

  10. Title? Method used? Legend? Are all panels relevant? Is the Figure relevant? Conclusion? Example-Figure1

  11. WT KO WT KO Emx2 Mash1 Dlx1 Gad1 SMRT normally prevents neuronal differentiation Method: In situ hybridization on brain sections at embryonic Day 12.5 Markers of differentiated neurons were increased in SMRT KO brains

  12. Black letters Yellow letters Packaging Use Arial or Helvetica bold Front 24-44 Use animation *not too much*

  13. Delivery Stand up Look at the audience Speak loudly + articulate Display enthusiasm Practice makes perfect

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