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Education and High School Outreach:

Collaborative Research: Mechanisms of Hybridization Kinetics in DNA Surface Layers Rastislav Levicky, Polytechnic University, DMR-0706170.

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Education and High School Outreach:

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  1. Collaborative Research: Mechanisms of Hybridization Kinetics in DNA Surface LayersRastislav Levicky, Polytechnic University, DMR-0706170 Surface DNA layers are extensively used in biomolecular diagnostics, from gene expression and genotyping to identification of forensic specimens and pathogen strains, yet many aspects of their physical behavior remain mysterious. This lack of fundamental understanding seriously impairs applications in clinical, research, and biothreat detection settings. Under “competitive” conditions typical of applications, thousands of solution nucleic acid strands with differing sequences compete for hybridization to a given surface-bound strand. This research seeks a detailed experimental and theoretical picture of competitive hybridization that is essential to effective experimental design and interpretation of these widely implemented, highly versatile diagnostic tools. TopLeft. Surface hybridization. Sample is shown in blue and immobilized strands in grey. Ferrocene tags (yellow star), conjugated to the sample strands, are used for quantitative electrochemical monitoring of the hybridization reaction. Top Right. Experimental data of surface hybridization conversion x as a function of ionic strength CB and immobilized DNA coverage SP. We are studying the kinetic pathways that lead to these final stationary states.

  2. HZ RG RB DG PG RL KW CC NT DI Collaborative Research: Mechanisms of Hybridization Kinetics in DNA Surface LayersRastislav Levicky, Polytechnic University, DMR-0706170 Education and High School Outreach: We are fortunate to have had a number of excellent participants contribute to this project through summer research including high school students Danny Zheng and Breveenn Kukan and first-year undergraduate Reju George. The project is also providing the main thesis focus for graduate students Dr. Damion Irving and Dongbiao Ge, as well as lecture material for the upcoming course Biointerfacial Engineering that will be developed by the P.I. BK Top Right. The PI (RL) with his group in summer 2007. Undergraduate student Reju George (RG) and graduate student Damion Irving (DI) were supported by this award. Bottom Right. The PI with high school student Breveenn Kukan (BK) during poster exhibition of summer research activities (also pictured is Prof. Charles Martucci (CM)). RL CM

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