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Verticillium dahliae Spinach Seed Progress Report. June 2, 2009. Seed Industry Collaborators. California Seed Association American Seed Trade Association (ASTA) ASTA - VegTech Subcommittee Puget Sound Seed Growers Association Researchers National Seed Health System
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Verticillium dahliaeSpinach SeedProgress Report June 2, 2009
Seed Industry Collaborators • California Seed Association • American Seed Trade Association (ASTA) • ASTA - VegTech Subcommittee • Puget Sound Seed Growers Association • Researchers • National Seed Health System • International Seed Health Initiative (ISHI Veg)
Seed Industry Presenters • Betsy Peterson – CSA • Philip Brown – Alf Christianson Seed • Tom Moore - Enza Coastal • Samantha Thomas - Seminis • Todd Rehrman – Syngenta Seed
Progress Highlights • Communication Established • Production Practices • Information Sheet • Research Activities • Seed Testing • Product Registrations • Seed Treatments/Processes
Communications • Meetings Leafy Greens/ Seed Industry • August 2008 – Salinas • March 16, 2009 – prior to CLGRB meeting • Industry Conferences • Industry / Researcher Roundtables • Conference Calls
SPINACH “A Challenging Crop”
Research and DevelopmentFrom Breeding to Sales • Year • 1-5 Selecting Lines: male – female • 6 Control on sex expression and purity • 6 Trial combinations • 6 Parent line propagation (trial) • 6 Trial seed production • 7 1st screening trial • 7 Parent line propagation (commercial) • 7 Seed production • 8 2nd screening • 9 Commercial
Product Development Specifications (Depending on market and type) • Disease resistances • Good leaf texture / high yield • Early – late maturity / vigor • Process ability: harvest to shelf life • Field stand ability: holding
Product Development Specifications (Depending on market and type) • Stands erect • Long Standing(Bolt resistance) • Uniformity / purity • Reliability / consistency
Spinach: Spinacia oleracia L. • Wind pollinated • Hybrid not OP • Isolation required • Hybrid seed production is based on: Dioecious (male) Monoecious (female)
Seed Production - Crop • Cool Climate • Very long days +/- 16 hours. • Good rotation 10-15 years (PNW), 5-6 years (EU). (Fusarium/Verticillium)
Seed Production - Grower • Grower Experience/Knowledge • Combines. • Seeds dryers • Close proximity of a seed conditioning facility.
Denmark 55˚N France 48˚N Italy 44˚N Washington 48˚N Oregon 44˚N Chile 40˚S Argentina 40˚S World WideSpinach Seed Production Locations New Zealand 46˚S
Information Sheet • “Fact Sheet” Developed compiled and distributed November 2008 • Literature Search • References Documented • Peer Reviewed, Version 3 out for review
Research Activities • Seed Industry $$ Funding • 2002 – present >$100,000 – Fusarium and Verticillium, duToit • Seed Company/Researcher Partnerships • Trial Funding • Seed Samples • Technical Assistance • Ring Tests • Industry Collaboration
Research Activities Continued • Recently Funded Research • ASTA (April 2009) • Lindsey duToit (WSU), Jim Correll (UAR) • $90,000 for 3 yrs. • Project has 4 objectives
ASTA Funded Project Objective 1: Examine the process of infection of spinach plants by V. dahliae from seedborne and soilborne inoculum, particularly with respect to earliness of bolting and development of Verticillium wilt symptoms.
ASTA Funded Project Objective 2: Assess the efficacy of conventional and organic seed treatments and soil drenches for protecting spinach against soilborne inoculum of V. dahliae (the efficacy against seedborne inoculum is being evaluated in the CLGRP project).
ASTA Funded Project Objective 3: Identify thresholds for soilborne vs. seedborne inoculum of V. dahliae for spinach cultivars with different levels of susceptibility to Verticillium wilt, and the influence of soil pH and soil type on V. dahliae inoculum potential from seedborne vs. soilborne sources.
ASTA Funded Project Objective 4: Determine the influence of soil microbial activity and soil fumigation on seed transmission, soil infestation, and development of Verticillium wilt as a result of planting spinach seed infected with V. dahliae
CDFA Specialty Crop Block Grant • Concept Submitted • June 4th Invitations to submit full proposal • June 29th Proposal Due Date
Potential Funding Sources • Specialty Crop Block Grant Program • USDA-CSREES- Specialty Crop Research Initiative • Technical Assistance for Specialty Crops (TASC)
Seed Testing • Partnerships - Seed Industry/Labs/ Researchers • Provide seed for testing • Provide $$ funding to lab/researchers • Coordinate & Participate in Ring Tests • Coordinate and Facilitate Training
Seed Testing Future • International Acceptance (VTS / NSHS / ISHI) • Accreditation • Coordinate Facilitate Workshops • Continuing Education
Product Registration • Types of Registrations • Section 3 • Section 24 (c) (Special Local Need (SLN) • Section 18 (Emergency Exemption) • Research Authorization RA
Section 18 or 24 (c ) SLN 24 (C) SLNSec 18 • Emergency No Yes • ResidueTolerance Yes No • Expiration No 1 year • *Grown for Seed Yes No * No part of seed plant enters food or feed
Product Registration Status • Topsin 24 (C) “grown for seed” WA State (approved February 2009) • Topsin Section 18 (Possibly?) • Mertect 340F Section 18 (Initiated Spring 2009)
Product Registration Status Con. • BASF 595 XGF (new a.i., no residue data) • Coronet (US EPA Reg in progress for leafy greens)
Section 18 Product InformationRequired • Authorization from Registrant • Description of Pesticide Requested • Name of Pest • Description of Proposed Use
Section 18 Data Required • Alternative Methods of Control • Efficacy • Food Quality Protection Act • Risk to Endangered or Threatened Species
Section 18 Economic Data Required • Identified Emergency • Discussion of Events or Circumstances • Significant Economic Loss
Section 18 Reporting Required • Interim Use Reporting • Progress Toward Registration
Section 18 Submission • Submit to CA DPR & WSDA • States Submit to US EPA • US EPA establishes time limited tolerance
Seed Treatments/Processes • AgriCoat • GTG • Incotec
Summary • Communication Established • Production Practices • Information Sheet • Research Activities • Seed Testing • Product Registrations • Seed Treatments/Processes
Thank You! Questions?