1 / 9

Real-Time Quantitative PCR

Real-Time Quantitative PCR. Jimmy Paden Jacci Tonn Melanie O’Callaghan. What is the Method?. Real-Time PCR delivers powerful capabilities for generating and analyzing data for a wide variety of applications. Highly sensitive quantification of transcriptional levels of the gene of interest.

fancy
Download Presentation

Real-Time Quantitative PCR

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Real-Time Quantitative PCR Jimmy Paden Jacci Tonn Melanie O’Callaghan

  2. What is the Method? • Real-Time PCR delivers powerful capabilities for generating and analyzing data for a wide variety of applications. • Highly sensitive quantification of transcriptional levels of the gene of interest

  3. How is it performed?

  4. What do you learn from such a method? • Can find out the quantity of genes that were started with • How many cycles it takes to make a certain number of genes • The less cycles means the more genes that were present at the beginning

  5. Why do this Method? • Faster • Can do it in a few hours not days • Quantitative

  6. DATA • http://www.ashi-hla.org/publicationfiles/ASHI_Quarterly/25_3_2001/The%20Usefullness%20of%20Quantitative.pdf

  7. References • http://www.ashi-hla.org/publicationfiles/ASHI_Quarterly/25_3_2001/The%20Usefullness%20of%20Quantitative.pdf • www.bio.davidson.edu/Courses/genomics/method/realtimepcr.html • www.dorak.info/genetics/realtime.html • www.dna.iastate.edu/frame_qpcr.html

More Related