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Modulation of C. elegans locomotory behavior by food

Modulation of C. elegans locomotory behavior by food. Tracks on Food. Well Fed Worm (off Food). Starved Worm (off Food). Gray et al , 2005. A dominant mutation in egl-4 causes excessive lipid accumulation. mg410 K162N. 48% identical 65% similar to human PKG-I . EGL-4. cGMP

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Modulation of C. elegans locomotory behavior by food

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  1. Modulation of C. elegans locomotory behavior by food Tracks on Food Well Fed Worm (off Food) Starved Worm (off Food) Gray et al, 2005

  2. A dominant mutation in egl-4 causes excessive lipid accumulation mg410 K162N 48% identical 65% similar to human PKG-I EGL-4 cGMP binding Kinase Auto-inhibition wild-type egl-4(gf)

  3. Modulation of C. elegans locomotory behavior wild-type egl-4(gf) sup 1 egl-4(gf); sup 1 Yan Hao

  4. Correlation between fat storage and locomotory behavior? wild-type kat-1 tub-1 kat-1 tub-1

  5. How do we make worm movies? Field of view = 1.5 x 1.5 cm E. coli length of a worm = 1mm

  6. What do we want to know from worm movies? • Posture of worms at rest and in motion Kindt et al, 2007 • Different behavioral states • in the presence or absence of food Fujiwara et al, 2002 • Speed of movement • Frequency of turns • Frequency of reversals Gray et al, 2005

  7. Visualization of head and tail sensory neurons by DiI Zeynep Altun and Dave Hall Wormatlas

  8. Assignment of head and tail of a worm in movies 1 second per frame 50 frames Zhou Xu

  9. Future directions • High resolution tracking coupled with motorized stage • Extended duration of tracking (>1 hour) • Live processing to reduce amount of stored data

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