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Prather Lab Lab Specific Training

Prather Lab Lab Specific Training. Kevin Solomon 4 February, 2009. Lab Safety Features. 2 Eyewash Stations 1 Safety Shower 1 Fire Extinguisher MSDS binder located on bookshelf by Lab Entrance 2 Emergency Exits. To Stata. To 56. To Ames St. Personal Protective Equipment.

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Prather Lab Lab Specific Training

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  1. Prather LabLab Specific Training Kevin Solomon 4 February, 2009

  2. Lab Safety Features • 2 Eyewash Stations • 1 Safety Shower • 1 Fire Extinguisher • MSDS binder located on bookshelf by Lab Entrance • 2 Emergency Exits To Stata To 56 To Ames St

  3. Personal Protective Equipment • Safety/Prescription glasses should be worn at all times • Be mindful of UV hazards near the EtBr area • Gloves should be worn most times • Protection of self from biohazards • Protection of experiments from human-hazards • Latex Examination Gloves serve only as water permeable barrier • Consult MSDS and Glove Manufacturers for permeability to hazardous reagents • Lab coats provided, but optional • Recommend wearing for handling of hazardous materials (e.g. Ethidium Bromide)

  4. Good Microbial Practices • Work with live cultures is permissible on open bench (BL1) • For sterility • Should be in Biosafety Cabinet, or • Near open flame on benchtop • Know the biohazard level of purchased strains & materials • BL2 or higher organisms pose additional risks for personnel and requires lab re-certification • Always wear gloves when working with cultures • Be mindful of glove contamination

  5. Waste Disposal • EHS, Federal Regulations and QuANGOs require the separation of waste streams according to hazard type • Solid Waste • Nonhazardous Waste • Recycling Bin • Solid Biohazardous Waste • Biosharps • Chem Sharps • EtBr Solid Waste

  6. Waste Disposal Cont’d • Liquid Waste • Sink • Qiagen/Zymo Waste • Guanidine Thiocyanate (QG/ADB) • Chemical Waste • Alkaline Aqueous Waste – For pH ≥ 7 solutions that are not oxidizing or predominately organic. • Acidic Aqueous Waste – For pH ≤ 7 solutions that are not oxidizing or predominately organic. • Organic Waste – For waste that is predominantly organic in nature (ethanol, acetone, butanol, etc.), except for waste containing strong oxidizers. • Oxidizer Waste – For waste containing any amount of a strong oxidizer (hydrogen peroxide, permanganate, chromates, nitric acid, etc.).

  7. Waste Disposal Guidelines • Treat liquid biowaste w/ 10% (v/v) bleach solution for 5+ mins before sewering • Check Sink Disposal sign under sink for chemicals that may be safely sewered • All liquid waste (including biohazardous garbage) must be kept in secondary containment at all times • All liquid waste must have a Red Tag • When waste is full, date the tag and call EHS for pickup • If a waste stream does NOT have a Red Tag, it is Effluent. Do not call it Waste • If you add to a waste stream, update the Red Tag (exception: Miniprep and QG/ADB Waste) • When in common areas, please empty and replace waste receptacles if full • Do not overflow HPLC Effluent • For Mixed Waste: Bio designation takes priority except with volatile organics • May need separate containment for mixed

  8. Miscellany • Break down boxes before recycling • Do not throw boxes or other garbage in the hall way (exception: Biosharps) • Storage of hazardous reagents should be in the solvent cabinet and not personal benches/shelves. • When new chemicals are introduced to the lab, obtain the MSDS and add to the binder • HOUSEKEEPING – more important as lab becomes more crowded.

  9. Final Notes • To be safe, you must be aware of your surroundings. • Assume any new chemical is unsafe until you find out otherwise. • Assume any material to be discarded is hazardous unless you know otherwise.

  10. EHS Rep Weekly Inspection • Occurs every Friday by our EHS Rep • See level 1 checklist at: http://web.mit.edu/environment/ehs/rep_tools.html

  11. Be CAREFUL and use COMMON SENSE! • KNOW what you are working with • LABEL – especially when using common areas • CLEAN up common areas immediately and periodically maintain your lab bench • Be RESPECTFUL of other people’s experiments, solutions, reagents, etc.

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