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Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit

Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit. HC 70AL SPRING 2009 APRIL 7, 2009 ANHTHU BUI. Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit. SRB Leaves Freezed in liquid nitrogen. Lysate. Adjust buffer conditions by

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Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit

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  1. Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit HC 70ALSPRING 2009APRIL 7, 2009ANHTHU BUI

  2. Isolation of Genomic DNA from Scarlet Runner Bean Leaves Using DNeasy Plant Mini Kit SRB Leaves Freezed in liquid nitrogen Lysate Adjust buffer conditions by adding AP3 buffer &ethanol Grind into powder with a mortar & pestle Separate DNA from contaminants using a silica-gel spin column Frozen SRB Leaf Powder Lyse cells with AP1 extraction buffer Genomic DNA, ribosomal RNAs, tRNAs, mRNAs, proteins, polysaccharides Genomic DNA bound to silica-gel column Digest RNAs with RNase A Wash column Centrifuge Centrifuge Elute with water or low-salt buffer Genomic DNA, proteins, oligo-ribonucleotides, polysaccharides Cell debris Pure genomic DNA Precipitate polysaccharides & proteins with AP2 buffer Filtered & homogenized via Shredder column Lysate

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