Formability of 3D tissue constructs

1. Formability of 3D tissue constructs

2. Characterization of 3D Tissue Constructs • Viability • Proliferation • Hepatocyte-Specific Function VS Standard 2D Monolayer Computer-Aided 3D Construct

3. Effect of Direct Cell Writing on cell viability • DECREASED Cell Viability Hepatocyte Cell Viability as a Function of DCW Process and Crosslinking Static 2D monolayer control Dynamic 3D bioprinted experimental group

4. Effect of Direct Cell Writing on cell proliferation • SIMILAR Cell Proliferation Media w/o cells Std Method cell suspension using conventional pipet

5. Effect of Direct Cell Writing on cell-specific function • IMPROVED Hepatocyte Specific Function

6. Analysis of Results Cell Viability Cell Cell Function Proliferation Resemble In Vivo Tissue: Importance: DCW 3D Constructs Offer: • Function/behavior • Differentiation • Proliferation • Cell-cell communication • Direct contact • Paracrine • ECM production from neighboring cell • Tight control of spatial dist. • High cell density • Co-culture multiple cell types

7. Applications as Pharmacokinetic Model Pic From: Computer-Aided Design, Modeling, and Freeform Fabrication of 3D Tissue Constructs for Drug Metabolism Studies