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Mycobacterium tusciae , sp. nov.

Mycobacterium tusciae , sp. nov. Enrico Tortoli. 19° ESM, Lisbon, July 22-25, 1998. Case report. 7-year old boy suffering from nephrotic syndrome from the age of 3 steroid treatment in the last 4 years cervical lymphadenitis surgical removal no relapse so far. Cultural features.

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Mycobacterium tusciae , sp. nov.

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  1. Mycobacterium tusciae, sp. nov. Enrico Tortoli 19° ESM, Lisbon, July 22-25, 1998

  2. Case report • 7-year old boy suffering from nephrotic syndrome from the age of 3 • steroid treatment in the last 4 years • cervical lymphadenitis • surgical removal • no relapse so far

  3. Cultural features • growth: slow (4 weeks) • growth temperature: 25°-32°C • pigmentation: scotochromogenic • colonies: rough

  4. Biochemical features • positive reactions: • nitrate reduction • thermostable catalase • urease • Tween 80 hydrolysis • 14-day arylsulfatase • acid phosphatase

  5. Antimicrobial susceptibility Drug MIC Ciprofloxacin  1 Clarithromycin  2 Ethambutol 8 Rifabutin  0.5 Rifampin  0.5 Sparfloxacin  0.5 Streptomycin  2

  6. Lipidic analysis • TLC: • -mycolates • keto-mycolates • wax esters • pattern shared by: M. scrofulaceum, M. interjectum, M. xenopi • Gas-chromatography: • 16:0 30% • 18:1 cis 9 21% • 18:0 alcohol 11% • 18:0 10-methyl 10% • pattern shared by M. scrofulaceum

  7. lmwIS hmwIS M. intracellulare FI-25796 HPLC

  8. 16S rDNA sequence • unique sequence both in hypervariable region A and B • short helix 18 • phylogenetic position among rapid growers, close to M. flavescens and M. smegmatis

  9. M. tusciae sp. nov. • Features of the type strain • acid fast rod • G + C content = 66.4 mol% • distinguishable from other scotochromogenic mycobacteria by means of biochemical tests • unique HPLC profile • not identifiable by TLC and GC alone • susceptible to all antimycobacterial drugs • unique sequence within 16S rDNA

  10. Conclusions • M. scrofulaceum is classically considered a major responsible of cervical lymphadenitis • M. tusciae adds to the scotochromogenic mycobacteria recently described as cause of pediatric lymphadenitis • part of pathologies attributed to M. scrofulaceum are probably due to misidentified novel mycobacteria

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