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Bioremediation Systems to Treat Nursery Runoff. Chris Wilson and Tom Yeager University of Florida/IFAS Joe Albano USDA/ARS Horticultural Research Lab. Objectives.
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Bioremediation Systems to Treat Nursery Runoff Chris Wilson and Tom Yeager University of Florida/IFAS Joe Albano USDA/ARS Horticultural Research Lab
Objectives • To evaluate the feasibility of adapting and using common aquaculture bioremediation technology for removing nutrients from nursery runoff water.
Objectives • Determination of conditions for optimization of: • Nitrification (Conversion of ammonia to nitrate) • Denitrification (Conversion of nitrate to nitrogen gas) • Phosphate precipitation
Process Requirements • Nitrification • Aerobic process • NH3/NH4+ to NO2-: Nitrosomonas, Nitrosoccus, Nitrospira, Nitrosolobus, Nitrosovibrio • NO2- to NO3-: Nitrobacter, Nitrococcus, Nitrospira, Nitrospina • Nitrifying bacteria are primarily obligate autotrophs, consuming CO2 as their primary C-source • Denitrification • Anaerobic/anoxic process • Nitrate serves as electron acceptor for oxidation of organic compound (sometimes inorganic) • Primarily organic C-source • Phosphate precipitation • Change from low redox potential to higher one can co-precipitate phosphate with iron and calcium
Project Stages • Phase 1: Pilot studies • Small scale • Determine optimal conditions • Phase 2 and 3: Scale up to nursery-size • Implement systems on working nurseries for evaluation
Reactor 2 Reactor 1 Sump Basic Reactor Design
Denitrification -media- -Low Redox- Phosphorus Precipitation -media- -High Redox- Nitrification -media- Controls -no media- Study Design Water Supply Canal
Primary variable for evaluation … • Water flow rates
Analytes • Nutrients • Nitrate and ammonia • Reactive phosphate • Biological Oxygen Demand • REDOX potential • pH • Dissolved oxygen • Select pesticides
Results • Performance characterization • Flow rate vs. removal efficiency/performance • Will aid in determining configuration for stage 2 project implementation
Denitrification Phosphorus Precipitation Nitrification Potential Impact