Introduction to the Microscope

1. Introduction to the Microscope Care Parts Focusing

2. BELL WORK ANSWER: • What is the function of a microscope? • How do you calculate for the power of magnification of a microscope?

3. STUDENTS WILL: • Know the parts of the microscope • Know the function of each parts of the microscope. • Know how to use and care for the microscope • Discuss the history of the microscope • Identify the types of microscope

4. VOCABULARY • EYEPIECE • NOSEPIECE • OBJECTIVE LENSES • STAGE CLIPS • COURSE ADJUSTMENT KNOB • FINE ADJUSTMENT KNOB • DIAPRAGHM • Resolution • Magnification

5. Microscope Care • Always carry with 2 hands • Only use lens paper for cleaning • Do not force knobs • Always store covered • Keep objects clear of desk and cords

6. Using the Microscope • Place the Slide on the Microscope • Use Stage Clips • Click Nosepiece to the lowest (shortest) setting • Look into the Eyepiece • Use the Coarse Focus

7. Using High Power • Follow steps to focus using low power • Click the nosepiece to the longest objective • Do NOTuse the Coarse Focusing Knob • Use the Fine Focus Knob to bring the slide What can you find on your slide?

8. What’s my power? To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.

9. Comparing Powers of Magnification • We can see better details with higher the powers of magnification, but we cannot see as much of the image.

10. Which of these images would be viewed at a higher power of magnification?

11. Microscope Parts Eyepiece BodyTube RevolvingNosepiece Arm ObjectiveLens Stage StageClips CoarseFocus Diaphragm FineFocus Light Base

12. TERMS TO KNOW… • ARM- used to support the microscope when it is carried. • 2. COURSE ADJUSTMENT KNOB- moves the stage up and down for focusing.

13. 3. FINE ADJUSTMENT KNOB- moves the stage slightly sharpen the image. 4. DIAPHRAGM- regulates the amount of light on the specimen. 5. BASE- supportsthe microscope

14. 6.EYEPIECE-allows you to view the image on the stage and contains the ocular lens. 7. NOSEPIECE- holds the HIGH –and LOW- power objective lenses; can be rotated to change magnification. 8. OBJECTIVE LENSES- found on the nose piece and and range from low to high power. (10x to 40x)

15. 9. STAGE CLIPS- hold the slide in place 10. LIGHT SOURCE- projects light upwards through the diaphragm, the specimen, and the lenses. 11.STAGE- supports the slide being viewed. 12. Body Tube – holds and support the eyepiece

16. Microscope Notes

17. Microscope One or more lense that makes an enlarged image of an object.

18. History of the Microscope • 1590 –first compound microscope

19. Around 1590 Zaccharias and Hans Janssen experimented with lenses in a tube, leading to the forerunner of the microscope and the telescope

20. History of the Microscope • 1655 – Robert Hooke used a compound microscope to observe pores in cork • He called them “cells”

22. History of the Microscope • Antoine van Leeuwenhoek • 1st to see single-celled organisms in pond water

23. Microscope Vocabulary • Magnification: increase of an object’s apparent size • Resolution: power to show details clearly • Both are needed to see a clear image

24. Types of Microscopes • Simple • Compound • Electron

25. Simple Microscope 1. Similar to a magnifying glass and has only one lense.

26. Types of Microscopes • 2. Compound Light Microscope • 1st type of microscope, most widely used • light passes through 2 lenses • Can magnify up to 2000x

27. Ocular lens Objective lenses

28. Types of Microscopes • 3. Electron Microscope • Used to observe VERY small objects: viruses, DNA, parts of cells • Uses beams of electrons rather than light • Much more powerful

29. Types of Electron Microscopes A Transmission Electron Microscope (TEM) • Can magnify up to 250,000x

32. Types of Electron Microscopes B. Scanning Electron Microscope (SEM) • Can magnify up to 100,000x