Welcome to Bio181L!

1. Welcome to Bio181L! • Contemplating the nuts & bolts of Life • xVivo’s 3D cell animation • (also on TA desktop--Lab01TaoTA => Biovisions => BioVisionsPlayer.swf)

2. About me... • Kelsey Berg • kberg@email.arizona.edu • Majors in Physiology/Molecular and Cellular Biology, Minor in Chemistry • From Phoenix, Arizona

3. What 181L is • Thinking, Understanding, Investigating, Evaluating • Not arrive, assembly line, leave • Not synchronized with most lecture sections, but internally coherent & mutually reinforcing

4. Science and this course • “One of the beautiful things about science is that it allows us to bumble along, getting it wrong time after time, and feeling perfectly fine as long as we learn something each time.”

5. About you Last name, First name Section # Year Major(s) Minor(s) What do you want to do (career)? Where are you from? What do you expect from this course? Anything else that I should know about you (optional)? • NOTE: The section dance is now over • Once a lab has met, no one can change into it

6. About the manual • Reading it will prepare you for class & in-class quizzes • Reading prior to 5’ before class will allow you to understand & think about what you’re reading • Lays out Philosophy (vii), Learning Goals (ix), expectations (xi) • Contains Periodic Table (0-1) and Molecular Basics (0-3), rules of molecule pics (0-5) and a cell (0-7)

7. 181 Lab ACCOUNTS • http://blc.arizona.edu/courses/181lab • Click ‘Create Accounts’ • Your section number is: XX • Bio181L_Go is the best way to do your work. Browser not so much

8. 80% of success is just showing up* • Absences must be excused from from Asya Roberts in BSE109 prior to making up • Arriving late is absent. Leaving early is absent. • Labs cannot be offered week after they are delivered; Avoid missing a lab • Missing 2 labs blows a huge hole in the intellectual content. You may be dropped from the course--excused or otherwise *--Woody Allen

9. My Webpage • Get there via homepage* => Instructors => (Section #) *http://blc.arizona.edu/courses/181Lab (Lab Man yellow page)

10. Web page operation

11. What to expect • Homework is serious--start early, and work in bursts, with gaps. Generally, 2-3 components/week • You will play a major role in experiment, protocol design • i.e., you’ll need to know what you’re trying to achieve rather than showing up & following steps. Sometimes, there aren’t any

12. Policies & Grading • Syllabus (Linked on course homepage) • Honor code & Plagiarism (Manual, p. xiii) • Assignments • On-line assessments/tutorials (10%) • In-class quizzes (15%) • Lab assignments (LABAs--Lab Activity Based Assignments) (50%) • Lab Projects & reports (combine to 25%)

13. How? Why? the Tao of Molecules • How molecules feel & the world they live in

14. Primary goals • Create understanding by observation, reasoning • Chemical foundations for the course: • Water & its properties • non-watery things • Know molecules as real & tangible things

15. When a journalist asked the great physicist Richard Feynman what single sentence would best encapsulate all science so far if it were to be the sole surviving scrap of all we knew, he replied,“The world is made of atoms.” From The Secret of Scent, Luca Turin p.28

16. Atoms: They’re how life works • DNA, RNA: C, H, N, O, P, [Mg++] • Carbs: C, H, O • Protein: C, H, N, O, (S), (P), [traces] • Membranes: C, H, N, O, P, (S) What cannot be done with assemblages of these atoms* cannot be doneby living organisms, nor their cells, nor their spit, etc. *OK, fine, there’s the occasional role for Ca++, etc.

17. Who am I? • At birth, # protons = # electrons • Atoms seek completion, which means outermost electron set = 8 (hydrogen, helium it’s just 2) Freeman Fig. 2.1a

18. Coloring your world

19. + O H H + - Four views of Water (There will be a test) H2O See lab manual, p. 0-3

20. Interacting with H2O To your StructViewers! Desktop => Bio181L_Go

21. Doing it

22. Clean up! • Oil waste in the hood • ethanol waste in the sink • anything too messy wadded up and discarded

23. It’s a real term; used to refer to a situation where the addition of salt (portable charges) alters the solubility of other molecules in water Why should this be? “Salting out”

24. First things first--what is it? What’s going on in terms of molecules Experiment: Ethanol and water on your arm: which is cooler & why? Prediction: based on molecular weight, which should evaporate more quickly--H2O (2 x 1 + 1 x 16) or CH3CH2OH (5 x 1; 2 x 12; 1 x 16) If not, why not? Salt--ever tasted your sweat? Or anybody else’s for that matter? What benefit might there be to adding NaCl to water that you are intending to evaporate? Evaporation

25. Some advice http://www.damnlol.com/please-do-me-right-now-224.html

26. What’s an Assessor? =>Chatting<=

27. What’s vocabulary homework? • EITHER both versions of the crossword exercise • OR VocabuWary with a better than threshold score • For “Atoms & Molecules”, F12, that’s 60,000 • Errors count off. Slowness counts off.

28. Homework due 10 p.m. before lab Extensive! In Depth! Next week’s quiz will include Concepts from today Atom colors Deducing partial charges (from tutorial) Manual Ch. 2 • Assessor: 181 Intro ’12 • Vocab Atoms/Mols: xWord(x2) or ‘Wary • Assessor: Molecular World Tutorial