220 likes | 516 Views
The Effect of Different Levels of pH on the Fermentation of Yeast. By Rachel Koch. Hypothesis and Problem Statement. Problem: How do different levels of pH in the environment around yeast effect its fermentation ? Hypothesis:
E N D
The Effect of Different Levels of pH on the Fermentation of Yeast By Rachel Koch
Hypothesis and Problem Statement Problem: How do different levels of pH in the environment around yeast effect its fermentation? Hypothesis: If the environment around the yeast has a higher pH, then the yeast will create more CO2.
Background Research • Yeast: microorganism, Eukaryotic domain and the Fungi kingdom • Baker’s yeast: digests sugar • Fermentation: release carbon dioxide • Energy and warmth for fermentation • Dough rising is result of fermentation
Design Diagram • Title: The Effect of Different Levels of PH on the Fermentation of Yeast • Hypothesis: If the environment around the yeast has a higher pH, then the yeast will create more CO2.
Design Diagram cont. • IV: pH level • DV: Height of dough(cm) • Constants: Ingredients in dough (besides IV), the measurements of each ingredient, temperature at which the dough is placed to ferment and the amount of time the yeast ferments.
Materials • Flour • Sugar • Instant yeast • Warm water/solution • Oil • Salt • Ruler • HCl • NaOH • Oven
Procedure • Prepare pH solutions in lab with the instructor using NaOHfor basic solutions and HCl for acidic solutions. 1. Heat water/solution to 35 degrees C. 2. Add flour, sugar, oil, yeast and salt to the warmed water/solution.
Procedure cont. 3. Knead dough for about 8 minutes. 4. Measure the height of the dough. 5.Store dough in a warm place for 45 minutes to allow yeast to ferment.
Procedure cont. 2 • Put dough in the oven set to 54 degrees C and turn the oven off 6. Measure the final height that the dough raises with a ruler.
Averages • PH 5: 4.8 cm • PH 6: 2.8 cm • PH 7 (control): 4.2 cm • PH 8: 3.2 cm
Graph The Effect of Different Levels of pH on the Fermentation of Yeast Height of dough in cm. Level of pH
Statistical Analysis ANOVA
Conclusions • Hypothesis did not support data • Ideal environment is pH 4-6 • Yeast ferments better in acidic substances • Data is inaccurate
Possible Errors • Oven temp. wasn’t exactly the same every time • Measurements might not have been exact • Measured every half centimeter
Improvements • Could buy an alternative heating devise • Measure ingredients more carefully • Measure the height to a tenth of a cm
Reasoning • Like to bake • Thought it was interesting that yeast makes dough rise
Extensions • Test other factors that effect yeast fermentation, like: • Temperature • Amount of sugar
Acknowledgements • Mrs. P • Preparing solutions • Helping • Parents • Bought ingredients