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Suppl. H. 60. 50. 40. 30. 20. 10. 0. A. B. NC CR1. NC CR1. DMSO. D PP2 PP3 D PP2 PP3 5 m M , 2 h. a P-SFK(Y416). a Src. cells with neurites (>one cell body diameters) /100 adherent cells. a P-ERK1/2. a ERK1/2. PP2. Li NC CR1 CR2.
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Suppl. H 60 50 40 30 20 10 0 A B NC CR1 NC CR1 DMSO D PP2 PP3 D PP2 PP3 5mM,2 h aP-SFK(Y416) aSrc cells with neurites (>one cell body diameters) /100 adherent cells aP-ERK1/2 aERK1/2 PP2 Li NC CR1 CR2 NC CR1 D PP2 D PP2 DMSO PP2 PP3 WB: a pTyr aShcA PP3 PP2, PP3 5mM, 2h Inhibitor of Src family kinase abrogate neurite outgrowth and ShcA phosphorylation induced by ShcC RNAi. Cells transfected with ShcC siRNA were treated with PP2,a inhibitor of Src family kinases, PP3, an inactive derivative, or with DMSO, incubated for 2 h in the tissue culture condition, then harvested for western blot analysis or counted for neurite-containing cells. A. Activation of Erk and ShcA following collagen stimulation is suppressed by PP2, but not by PP3 or DMSO. B. Neurite outgrowth induced by ShcC siRNA was partially suppressed by PP2. IP: aShcA