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Infect TIVE with BCBL-1 virus. TIVE KSHV + day 7 Ad-orf50 day 4. SLK KSHV + Ad-orf50. TIVE KSHV + day 7. TIVE KSHV -. 4 days post Inf. LANA IFA. K8.1. Induce lytic cycle TPA 6 days. Purify virus from TIVE; infect fresh TIVE cells. LANA IFA 4 days post Inf. Repeat 3 times.
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Infect TIVE with BCBL-1 virus TIVE KSHV + day 7 Ad-orf50 day 4 SLK KSHV + Ad-orf50 TIVE KSHV + day 7 TIVE KSHV - 4 days post Inf. LANA IFA K8.1 Induce lytic cycle TPA 6 days Purify virus from TIVE; infect fresh TIVE cells LANA IFA 4 days post Inf. Repeat 3 times Suppl. Figure 2 A B C