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Urine sample handling

Urine sample handling. 1. Freeze @ -20ºC. Process. Spin. Thaw. 2. Freeze @ -20ºC. Process. Thaw. Spin. Pool individual urine samples. 3. Freeze @ -20ºC. Process. Spin. Thaw. Split into 4 identical samples. RT 4 hrs. 4. Freeze @ -20ºC. Process. Thaw. Spin. 12. 10.

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Urine sample handling

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  1. Urine sample handling 1 Freeze @ -20ºC Process Spin Thaw 2 Freeze @ -20ºC Process Thaw Spin Pool individual urine samples 3 Freeze @ -20ºC Process Spin Thaw Split into 4 identical samples RT 4 hrs 4 Freeze @ -20ºC Process Thaw Spin

  2. 12 10 8 6 4 2 0 0.0 0.2 0.4 0.6 0.8 1.0 Total # of feature: 15030 S/F/T vs. F/T/S RT/S/F/T vs. RT/F/T/S Density 0.0 0.2 0.4 0.6 0.8 1.0 Tukey HSD P value between sub groups

  3. S/F/T vs. F/T/S RT/S/F/T vs. RT/F/T/S P (Two-Tailed) = 0.013 -3 < t < 3 t < -3 t > 3 t < -3 -3 < t < 3 t > 3 122 14825 83 196 14762 72 4 Randomize Feature set 20 X MW/10000 2 0 3 2 # X 100 1 0

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