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Supplementary Figures

Supplementary Figures. Supplementary Figure S1. siControl. pcDNA3. siControl. pcDNA3. BRCA1wt. siBRCA1. BRCA1wt. siBRCA1. MDA-MB-231. Proliferation. A. *. ***. 120. ***. **. *. ***. 100. **. ***. ***. ***. 80. ***. ***. cells in S+G2/M (% of control). 60. 40. 20.

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Supplementary Figures

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  1. Supplementary Figures

  2. Supplementary Figure S1 siControl pcDNA3 siControl pcDNA3 BRCA1wt siBRCA1 BRCA1wt siBRCA1 MDA-MB-231 Proliferation A * *** 120 *** ** * *** 100 ** *** *** *** 80 *** *** cells in S+G2/M (% of control) 60 40 20 0 C DEX DEX+AG C DEX DEX+AG B Apoptosis *** * *** 120 ** ** *** ** 100 *** 80 *** cells in SubG1 (% of control) 60 40 20 0 C DEX DEX+AG C DEX DEX+AG

  3. siControl pcDNA3 BRCA1wt siBRCA1 Supplementary Figure S2 MDA-MB-231 A B *** *** *** 140 *** 250 *** 120 200 100 150 *** 80 GRE-Luc fold induction 100 60 50 40 *** * 1 1 *** 0.5 0.5 0 C DEX C DEX *** G0S8 *** MKP-1 5 *** 10 *** *** *** 4 8 *** *** *** 3 6 mRNA fold induction mRNA fold induction 2 4 1 2 *** 0 0 C DEX C DEX C C DEX C DEX C DEX C DEX C DEX C DEX siControl siBRCA1 pcDNA3 BRCA1wt pcDNA3 BRCAwt siCtl siBRCA1 IEX-1 tPa 1.2 1.2 *** *** *** *** 1.0 1.0 0.8 0.8 *** *** *** 0.6 0.6 *** mRNA fold induction mRNA fold induction *** 0.4 0.4 *** 0.2 0.2 0 0 C DEX C DEX C DEX C DEX C DEX C DEX C DEX C DEX siControl siBRCA1 pcDNA3 BRCA1wt siControl siBRCA1 pcDNA3 BRCA1wt

  4. Ø DEX BRCA1 wt BRCA1 wt pcDNA3 pcDNA3 Supplementary Figure S3 MDA BRCA1 silencing A B C Ø DEX si Control si BRCA1 siBRCA1 1.2 siBRCA1 siControl siControl 1 0.8 BRCA1 *** mRNA fold induction 0.6 *** GR P-Ser211 0.4 GR 0.2 Actin 0 BRCA1 GR BRCA1 overexpression D E F si Control si BRCA1 *** 5 BRCA1wt 5 pcDNA3 *** *** 3 3.5 *** *** *** 4 4 3 2.5 3 3 2.5 *** 2 6000 hGR 1B/C-Luc fold induction 2 *** hGR 1A-Luc fold induction BRCA1 2 2 2 hGR 1A-Luc fold induction 1.5 1.5 ** hGR 1B/C-Luc fold induction GR P-Ser211 ** 4000 1.5 GR mRNA levels BRCA1 overexpression condition/Ctl condition 1 GR 1 1 1 * BRCA1 mRNA levels BRCA1 overexpression condition/Ctl condition 1 ** Actin * * 2000 0 0 0.5 0.5 C DEX 0.5 C DEX 0 0 0 0 0h 12h 24h 36h 48h 0h 12h 24h 36h 48h C DEX C DEX

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