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Genomic Biotech for Marine Population Monitoring: Phylogeography Insights From mtDNA

Explore genomic phylogeography and connectivity in marine populations using complete mtDNA genomes. Discover genetic insights from DNA sequencing experiments and microarrays. Analyze population structures, migration events, and genetic consequences of environmental disruptions.

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Genomic Biotech for Marine Population Monitoring: Phylogeography Insights From mtDNA

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  1. Genomic Biotech for Environmental Monitoring: Phylogeography & connectivity among marine populationsas inferred from complete mtDNA genome sequencesSteven M CarrTerra Nova Genomics, Inc.&Dept of Biology, Memorial University of NewfoundlandSt John's NL, Canada2nd International Conference on OceanographyLas Vegas NV, 21 July 2014

  2. Two Genomes nucDNA genome 2,850,000,000 bp 20,500 genes mtDNA genome ~17,000 bp 38 genes

  3. DNA Sequencing on a MicroarrayComplete mtDNA genome in one experiment[ SM Carr et al. 2008. Comp BiochemPhysiol D 3:1-11 ]

  4. Molecular Velcro: Multi-species ArkChip(Three fish, three mammals, one bird: 115,984 bp)

  5. Information content of DNA microarrays:7 species X80 individuals / sppX 16.5 Kbp @ = 9.2 Mbp

  6. Atlantic Wolffish (Anarhicas lupus) Newfoundland Caribou (Rangifertarandus) Atlantic Cod (Gadus morhua) Harp Seals (Pagophilus groenlandicus)

  7. Harp Seal pupping & breedinggrounds White Sea Gulf Greenland Sea Front

  8. Genomic Phylogeography of Pagophilus16.5 Kbp @ from 83 Harp seals[ Carr, Duggan, Stenson, & Marshall2014 PLoS ONE, in review ]

  9. Is the observed tree geographically structured? White Sea Greenland Sea Ice Front Gulf Front ‘99 Stronger geographic structures require fewer migration events; Compare observed tree versus 10,000 random trees

  10. Are populations geographically structured ?Isolation-by-Distance YES: Trans-Atlantic “Stepping-stone” model requires significantly fewer migration events than random expectation

  11. Population expansion wrt Climate Change over 300 Ky Wisconsinan glaciation

  12. Testing structure of populations within speciesKolmogorov – Smirnov non-parametric test:Are cumulative genetic distance curves significantly offset? YES: Diversity among Greenland Sea Harp Seals is younger than among White Sea seals [ versus]

  13. Spawning Stocks of Atlantic CodAre they genetically distinguishable populations ?

  14. Family Tree of Atlantic Cod:Northern Cod (2J3KL) vsTrans-Laurentian stocks (4WX5Ze)vsFlemish Cap (3M) vsNorwegian Coastal stocks[Carr & Marshall 2008 Genetics180,381& in progress ] Bay Cod

  15. Worst-Case Scenario:Oil Spill impacts Genome Ecosystem

  16. Genetic Consequences of Environmental Disruption Gene Flow Local success & dispersal Loss of connectivity: disruption of gene flow & dispersal

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