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Two Genomes

gabriel-merritt
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Two Genomes

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  1. Genomic Biotech for Environmental Monitoring: Phylogeography & connectivity among marine populationsas inferred from complete mtDNA genome sequencesSteven M CarrTerra Nova Genomics, Inc.&Dept of Biology, Memorial University of NewfoundlandSt John's NL, Canada2nd International Conference on OceanographyLas Vegas NV, 21 July 2014

  2. Two Genomes nucDNA genome 2,850,000,000 bp 20,500 genes mtDNA genome ~17,000 bp 38 genes

  3. DNA Sequencing on a MicroarrayComplete mtDNA genome in one experiment[ SM Carr et al. 2008. Comp BiochemPhysiol D 3:1-11 ]

  4. Molecular Velcro: Multi-species ArkChip(Three fish, three mammals, one bird: 115,984 bp)

  5. Information content of DNA microarrays:7 species X80 individuals / sppX 16.5 Kbp @ = 9.2 Mbp

  6. Atlantic Wolffish (Anarhicas lupus) Newfoundland Caribou (Rangifertarandus) Atlantic Cod (Gadus morhua) Harp Seals (Pagophilus groenlandicus)

  7. Harp Seal pupping & breedinggrounds White Sea Gulf Greenland Sea Front

  8. Genomic Phylogeography of Pagophilus16.5 Kbp @ from 83 Harp seals[ Carr, Duggan, Stenson, & Marshall2014 PLoS ONE, in review ]

  9. Is the observed tree geographically structured? White Sea Greenland Sea Ice Front Gulf Front ‘99 Stronger geographic structures require fewer migration events; Compare observed tree versus 10,000 random trees

  10. Are populations geographically structured ?Isolation-by-Distance YES: Trans-Atlantic “Stepping-stone” model requires significantly fewer migration events than random expectation

  11. Population expansion wrt Climate Change over 300 Ky Wisconsinan glaciation

  12. Testing structure of populations within speciesKolmogorov – Smirnov non-parametric test:Are cumulative genetic distance curves significantly offset? YES: Diversity among Greenland Sea Harp Seals is younger than among White Sea seals [ versus]

  13. Spawning Stocks of Atlantic CodAre they genetically distinguishable populations ?

  14. Family Tree of Atlantic Cod:Northern Cod (2J3KL) vsTrans-Laurentian stocks (4WX5Ze)vsFlemish Cap (3M) vsNorwegian Coastal stocks[Carr & Marshall 2008 Genetics180,381& in progress ] Bay Cod

  15. Worst-Case Scenario:Oil Spill impacts Genome Ecosystem

  16. Genetic Consequences of Environmental Disruption Gene Flow Local success & dispersal Loss of connectivity: disruption of gene flow & dispersal

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