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THE MICROSCOPE

THE MICROSCOPE . Use of the Microscope Lab Manual Exercise # 2. THE MICROSCOPE. THE COMPOUND MICROSCOPE. BASE. It contains: Off on switch Voltage regulator illuminator. STAGE. It supports the microscope slides and contains slide clips and mechanical stage. MECHANICAL STAGE.

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THE MICROSCOPE

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  1. THE MICROSCOPE Use of the Microscope Lab Manual Exercise # 2

  2. THE MICROSCOPE ALFONSO A. PINO MD.

  3. THE COMPOUND MICROSCOPE

  4. BASE • It contains: • Off on switch • Voltage regulator • illuminator

  5. STAGE • It supports the microscope slides and contains slide clips and mechanical stage

  6. MECHANICAL STAGE • It has 2 knobs for movement of the slides from left to right and front to back

  7. SUBSTANCE CONDENSOR • It has 3 parts: • blue filter • iris diaphragm • condenser lens

  8. Blue filter: • the short wavelength of the blue light provides maximum resolution • Iris diaphragm: • controls the amount of light passing from the slide source • Condenser lens: • collets and focuses the light from the illuminator onto the slide

  9. LENSES • They magnify the image • Objectives: they have 3 or 4 objectives • 4X scanner • 10X low power • 40X high dry • 100 X oil emersion lens • Ocular lens or eyepiece: • it is 10X and it can have a scale

  10. ADJUSTMENT KNOBS • They are for focusing • Coarse adjustment knob: • for locating and focusing objects (DO NOT USE for high magnification) • Fine adjustment knob: (small) • for focusing on higher powers knobs

  11. ARM • Supports the objective lens

  12. CARES OF THE MICROSCOPE • When moving the microscope, carry it with 2 hands (one hand to grip the arm and the other under the base • Lenses have to be clean with lens paper (to keep them free of oil and dust). • Do not use the coarse adjustment when focusing with the higher power objectives

  13. PROCEDURES • 1- Turn on the illuminator using the on/off switch • 2- Turn the nosepiece to bring the 4X objective (scanner) into position • 3- Raise the stage into its highest position • 4- Place a slide of the letter “e” in the slide clamp on the stage • 5- Turn the coarse adjustment knob to bring the “e” into focus • 6- Measure the field (the brightly lighted circle that you see when you look through the ocular lens) • 7- Center the ”e” in your field of view and then rotate the nosepiece to 10X • 8- Use the fine adjustment knob to focus until the image is sharp. Draw the image. Do not use the coarse adjustment • 10- Rotate the nosepiece until the 40X. Draw the image

  14. SQUAMOUS CELLS • Simple squamous peritoneum

  15. CUBOIDAL CELLS • Simple cuboidal kidney ALFONSO A. PINO MD.

  16. COLUMNAR CELLS • Simple columnar small intestine ALFONSO A. PINO MD.

  17. REMEMBER!GO TO THE TUTORING ROOM AND PRACTICE WITH MODELS

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