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D-glucose

(a). D-glucose. ( b ). GLK1 HXK1 HXK2. PDC1. glucose-6-phosphate. 2. PGI1. 1.5. fructose-6-phosphate. 1. Relative expression. PFK1 PFK2. 0.5. 0. fructose-1,6-bisphosphate. WT. sir2 D. hst1 D. sum1 D. FBA1. ( c ). TPI1. PDC1. glyceraldehyde-3-phosphate. DHAP. WT. sir2∆.

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D-glucose

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  1. (a) D-glucose (b) GLK1 HXK1 HXK2 PDC1 glucose-6-phosphate 2 PGI1 1.5 fructose-6-phosphate 1 Relative expression PFK1 PFK2 0.5 0 fructose-1,6-bisphosphate WT sir2D hst1D sum1D FBA1 (c) TPI1 PDC1 glyceraldehyde-3-phosphate DHAP WT sir2∆ TDH3 TDH1 TDH2 hst1∆ sir2∆ hst1∆ 3-phospho-D-glyceroyl-phosphate sum1∆ Relative expression PGK1 3-phosphoglycerate GPM1 3 4 5 6 7 8 9 10 2-phosphoglycerate hrs post log phase ENO2 ENO1 (d) phosphoenolpyruvate PDC1 WT CDC19 PYK2 sir2∆ hst1∆ pyruvate sir2∆ hst1∆ sum1∆ PDC1 PDC5 PDC6 Relative expression acetaldehyde ADH1 ADH3 ADH4 ADH5 ALD6 ALD2 ALD3 ALD4 ALD5 7 8 9 10 ethanol hrs post log phase acetate

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