1 / 5

F plasmid integrates into chromosome by recombination

Donor chromosome. F + cell. 1. F plasmid integrates into chromosome by recombination. Hfr cell. Donor chromosome. F + cell. 1. F plasmid integrates into chromosome by recombination. Hfr cell. Pilus. 2. Cells join via a conjugation pilus. F + cell. F – recipient. Donor chromosome.

gaurav
Download Presentation

F plasmid integrates into chromosome by recombination

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell

  2. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell Pilus 2 Cells join via a conjugation pilus F+ cell F– recipient

  3. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell Pilus 2 Cells join via a conjugation pilus F+ cell F– recipient Copy of donor DNA F plasmid Part of F plasmid 3 F plasmid partially replicates and moves into recipient cell trailing a copy of donor’s DNA

  4. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell Pilus 2 Cells join via a conjugation pilus F+ cell F– recipient Copy of donor DNA F plasmid Part of F plasmid 3 F plasmid partially replicates and moves into recipient cell trailing a copy of donor’s DNA Incomplete F plasmid; cell remains F– 4 Conjugation ends with pieces of F plasmid and donor DNA in recipient cell Hfr cell F+ cell

  5. Donor chromosome F+ cell 1 F plasmid integrates into chromosome by recombination Hfr cell Pilus 2 Cells join via a conjugation pilus F+ cell F– recipient Copy of donor DNA F plasmid Part of F plasmid 3 F plasmid partially replicates and moves into recipient cell trailing a copy of donor’s DNA Incomplete F plasmid; cell remains F– 4 Conjugation ends with pieces of F plasmid and donor DNA in recipient cell Hfr cell F+ cell 5 Donor DNA and recipient DNA recombine making a recombinant F– cell Recombinant cell

More Related