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Unsatisfactory Newborn Screening Specimens: Interpretations, Studies and Current Trends

Unsatisfactory Newborn Screening Specimens: Interpretations, Studies and Current Trends. Presenters Dr. John Sherwin-California Health Department Dr. Ken Pass-New York Health Department Dr. Lisa Kalman-Center for Disease Control. The web conference is co-sponsored by.

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Unsatisfactory Newborn Screening Specimens: Interpretations, Studies and Current Trends

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  1. Unsatisfactory Newborn Screening Specimens: Interpretations, Studies and Current Trends • Presenters • Dr. John Sherwin-California Health Department • Dr. Ken Pass-New York Health Department • Dr. Lisa Kalman-Center for Disease Control

  2. The web conference is co-sponsored by • The Newborn Screening Quality Assurance Program (CDC) • APHL's Quality Assurance/ Quality Control/ Proficiency Testing Subcommittee of the Newborn Screening and Genetics in Public Health Committee • The National Laboratory Training Network

  3. Objectives • Pretest-assess the current status of evaluating dried blood spot specimens • Review of testing of inadequate specimens and equivalent adequate specimens • Summary of inadequate specimen handling

  4. We are going to show you a series of dried blood spot specimens. Through live online audience participation, please determine the suitability for testing of each specimenby clicking on the single best assessment in each of the polling windows. Results will follow each polling.

  5. Specimen # 1

  6. Poll: Specimen #1Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  7. Specimen #1 Assessment Uneven blood saturation- colored in circles with capillary

  8. Specimen #2

  9. Poll: Specimen #2Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  10. Specimen #2 Assessment Properly collected specimen

  11. Specimen #3

  12. Poll: Specimen #3Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  13. Specimen #3 Assessment Blood Clot on Specimen

  14. Specimen #4

  15. Poll: Specimen #4Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  16. Specimen #4 Assessment Circles not filled

  17. Specimen #5

  18. Poll: Specimen #5Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  19. Specimen #5 Assessment Poor saturation-possibly due to high hematocrit

  20. Specimen #6

  21. Poll: Specimen #6Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  22. Specimen #6 Assessment Contamination

  23. Specimen #7

  24. Poll: Specimen #7Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  25. Specimen #7 Assessment Incomplete saturation- too little blood applied

  26. Specimen #8

  27. Poll: Specimen #8Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  28. Specimen #8 Assessment Smeared blood- Pressed too hard against the heel

  29. Specimen #9

  30. Poll: Specimen #9Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  31. Specimen #9 Assessment Improper use of capillary tube - blood clotted before application causing serum rings

  32. Specimen #10

  33. Poll: Specimen #10Is this specimen satisfactory for testing? • Yes • No, blood is clotted • No, blood is layered • No, blood is contaminated • No, capillary tube was used to spot the blood • No, incomplete saturation • No, other

  34. Folds and creases through the blood circles Specimen #10 Assessment

  35. The dried blood spot specimens for the polling slides were generously provided by Gary HoffmanWisconsin State Laboratory of Hygiene

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