The Time Machine:

1. The Time Machine: Transforming Molecular Signals into Long-Lasting Synaptic Change David Santucci Quantitative Biology Bootcamp 2009

2. Presynaptic Release Long Term Potentiation Difference in time scale > 6 orders of magnitude

4. Induction Expression Early Late

5. LTP Induction Protocols

7. Blocking NMDA Receptors Blocks LTP

8. Uncaging Ca2+ Alone Can Induce LTP

9. Long Term Depression

11. Prot ProtK P P Prot Protein Kinases Catalyze the Phosphorylation of Proteins

12. P P CaMKII

13. Induction Expression Early Late

14. LTP Leads to an Increase in Surface AMPA Receptors

15. LTP Leads to an Increase in Spine Size

16. Induction Expression Early Late

18. Blocking Protein Synthesis Blocks the Late Phase of LTP

19. LTP TAG Prot

20. Weak Stimulus Followed by a Strong Stimulus Produces LTP

23. Molecular Makeup of the PSD

24. Scaffolding Molecules

25. PSD Proteins Are In Flux

26. Actin Treadmilling, Capping, Cutting and Cross-Linking

27. Actin Treadmilling, Capping, Cutting and Cross-Linking

29. QDOT AB AMPA Single-Particle Tracking of AMPA Receptor Diffusion

32. Imaging of Isolated PSDs with Immunogold EM

33. EM Tomography of Molecular-Scale Structures in the PSD

35. Förster Resonance Energy Transfer (FRET)

37. 488 nm 507 nm 405 nm 555 nm 572 nm Bleached Dendra2 Super-Resolution Imaging Using FPALM

38. 488 nm 507 nm 405 nm 555 nm 572 nm Super-Resolution Imaging Using FPALM

39. Super-Resolution Imaging Using FPALM

41. Thanks to QB Program Jané Kondev Jeff Gelles Trisha Murray John Lisman Sri Raghavachari All of you...