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Characterizing growth in broth

Characterizing growth in broth. Planning for August 2014 and onward. Current P roject Status. Interesting results from summer experiments Goal: grow cells that convert caffeine to theophylline in tetracycline broth August: replicate Shannon Doherty’s growth of JM109 #23 in broth

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Characterizing growth in broth

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  1. Characterizing growth in broth Planning for August 2014 and onward

  2. Current Project Status • Interesting results from summer experiments • Goal: grow cells that convert caffeine to theophylline in tetracycline broth • August: replicate Shannon Doherty’s growth of JM109 #23 in broth • One day @ 37°C and three days @ room temperature • Pilot study for incubation only at RT • Control for tetracycline function • Characterize growth of ΔNEP1:#23 cells Shannon Doherty, Erika Keffeler, Jon Lim

  3. Looking forward • Goal: expand knowledge on growth in tetracycline broth • Continue growing cells in various concentrations of caffeine • Grow cells exclusively at RT • Investigate growth of ΔNEP1 with other genotype combinations Shannon Doherty, Erika Keffeler, Jon Lim

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