Understanding Gas Exchange and Ventilation in Mammalian Respiratory System

1. Gas Exchange-topic 6.4 and -H 6

2. Ventilation • Ventilation:The flow of air in and out of the alveoli is called ventilation • two stages: • inspiration (or inhalation) and expiration (or exhalation).

3. Why do we need it ? • to maintain concentration gradients in the alveoli. • Diffusing O2 and CO2

4. Respiratorymedium(air of water) O2 CO2 Respiratorysurface Organismal level Circulatory system Cellular level Energy-richmoleculesfrom food ATP Cellular respiration Gas exchange occurs across specialized respiratory surfaces • Gas exchange-diffusion • Supplies oxygen for cellular respiration and disposes of carbon dioxide

5. Animals require large, moist respiratory surfaces for the adequate diffusion of respiratory gases • Between their cells and the respiratory medium, either air or water

6. Branch from thepulmonaryartery(oxygen-poor blood) Branch from the pulmonary vein (oxygen-rich blood) Terminal bronchiole Nasalcavity Alveoli Pharynx Left lung Esophagus Larynx 50 µm Trachea 50 µm Right lung Bronchus Bronchiole Colorized SEM SEM Diaphragm Heart Mammalian Respiratory Systems: A Closer Look • A system of branching ducts • Conveys air to the lungs

7. In mammals, air inhaled through the nostrils • Passes through the pharynx into the trachea, bronchi, bronchioles, and dead-end alveoli, where gas exchange occurs

8. Draw and label a diagram of the ventilation system, including trachea, lungs, bronchi, bronchioles and alveoli, diaphragm

9. Features of Alveoli • Large surface area (600 million alveoli = 80 m2) • Flattened epithelial cells of alveoli and close association with network of capillaries • Short diffusion distance from alveoli to blood (0.5-1.0 um) • Moist surface for the solution of gases • Warm as blood supply 

11. Alveoli

13. Breathing ventilates the lungs • The process that ventilates the lungs is breathing • The alternate inhalation and exhalation of air • Is needed to maintain high concentration gradient in the alveoli.

14. Rib cage expands asrib muscles contract Rib cage gets smaller asrib muscles relax Air inhaled Air exhaled Lung Diaphragm INHALATIONDiaphragm contracts(moves down) EXHALATIONDiaphragm relaxes(moves up) How a Mammal Breathes • Mammals ventilate their lungs • By negative pressure breathing, which pulls air into the lungs

15. Inhalation • Diaphragm contracts- flattens-downwards • External intercostal muscles contract. • Ribs move up and out • Volume in chest/thoracic cavity/lung increases • Pressure decrease • Air rushes in

16. Exhalation • Diaphragm relaxes-moves up-curved up • External muscle relaxes • Decrease in lung volume • Increase in pressure • Air is rushed out

17. During exercise • Abdominal muscles, • Intercoastal muscles contracts • Faster exhalation

19. Partial pressure-each gas has a partial pressure which is the pressure which the gas would have if it alone occupied the volume. • Units –k Pa-(kilo Pascal), mm Hg

22. No. of membrane it has to cross

23. Cerebrospinalfluid The medulla’s control center also helps regulate blood CO2 level. Sensorsin the medulla detect changes in the pH (reflecting CO2 concentration) of the blood and cerebrospinal fluid bathing the surface of the brain. 1 The control center in the medulla sets the basicrhythm, and a control centerin the pons moderates it,smoothing out thetransitions between inhalations and exhalations. 5 Nerve impulses relay changes in CO2 and O2 concentrations. Other sensors in the walls of the aortaand carotid arteries in the neck detect changes in blood pH andsend nerve impulses to the medulla. In response, the medulla’s breathingcontrol center alters the rate anddepth of breathing, increasing bothto dispose of excess CO2 or decreasingboth if CO2 levels are depressed. Pons Breathing control centers Nerve impulses trigger muscle contraction. Nervesfrom a breathing control centerin the medulla oblongata of the brain send impulses to thediaphragm and rib muscles, stimulating them to contractand causing inhalation. 2 Medullaoblongata Carotidarteries Aorta In a person at rest, these nerve impulses result in about 10 to 14 inhalationsper minute. Between inhalations, the musclesrelax and the person exhales. The sensors in the aorta andcarotid arteries also detect changesin O2 levels in the blood and signal the medulla to increase the breathing rate when levels become very low. 6 Diaphragm Rib muscles Control of Breathing in Humans • The main breathing control centers • Are located in two regions of the brain, the medulla oblongata and the pons

24. Hemoglobin

26. Hemoglobin (Hb), a globular protein, is the primary vehicle for transporting oxygen in the blood. • To a much lesser degree-blood's plasma • Each Hb molecule has the capacity to carry four oxygen molecules.

27. Each Hb molecule can join with 4 oxygen molecules • The increasing affinity for oxygen allows the haemoglobin to rapidly saturate in the high partial pressures of oxygen of the alveoil capillaries. • Color changes-red---dark red—reddish purple

28. The normal range of values for hemoglobin content • Female: 11.5-15.5 g /dl • Male: 13.5-17.5 g/dl

29. The amount of oxygen bound to the Hb at any time is related, • to the partial pressure of oxygen to which the Hb is exposed. • In the lungs, at the alveolar–capillary interface, the partial pressure of O2 is typically high, and therefore the oxygen binds readily to Hb that is present.

30. oxygen saturation. • How much of that capacity is filled by oxygen at any time is called the oxygen saturation. • Expressed as a percentage

31. Oxygen dissociation curves. • Important tool for understanding how our blood carries and releases oxygen. • relates oxygen saturation and partial pressure of oxygen in the blood (pO2), and is determined by what is called “Hb's affinity for oxygen”; • how readily Hb acquires and releases oxygen molecules into the fluid that surrounds it.

32. As the blood circulates to other body tissue in which the partial pressure of O2 is less, the Hb releases the O2 into the tissue because the Hb cannot maintain its full bound capacity of O2 in the presence of lower O2 partial pressures

33. O2 dissociation curve

35. The effect of carbon dioxide in the blood Hb can also bind carbon dioxide, but to a lesser extent. Carbaminohaemoglobin forms. Some carbon dioxide is carried in this form to the lungs from respiring tissues.

36. The presence of carbon dioxide helps the release of oxygen from Hb, this is known as the Bohr effect.

40. There are 3 ways in which carbon dioxide is transported in the blood: • 1. DISSOLVED CO2 • more soluble in blood than oxygen • About 5 % of carbon dioxide is transported unchanged, simply dissolved in the plasma

41. 2. BOUND TO HAEMOGLOBIN AND PLASMA PROTEINS • Carbon dioxide combines reversibly with Hb to form carbaminohaemoglobin. • Carbon dioxide does not bind to iron, as oxygen does, but to amino groups on the polypeptide chains of Hb. • 10 % of carbon dioxide is transported bound to Hb and plasma proteins

42. 3. BICARBONATE IONS (HCO3- ) • the majority of carbon dioxide is transported in this way • CA-Carbonic anhydrase