1 / 4

Mitochondrial data from the dark ages (1989): Suillus mt-DNAs vary 3X in size, and the COI region varies about 5-fold

Mitochondrial data from the dark ages (1989): Suillus mt-DNAs vary 3X in size, and the COI region varies about 5-fold. The ITS region has become the de facto bar- coding region for ectomycorrhizal fungi for the following reasons:.

geraldine
Download Presentation

Mitochondrial data from the dark ages (1989): Suillus mt-DNAs vary 3X in size, and the COI region varies about 5-fold

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mitochondrial data from the dark ages (1989): Suillus mt-DNAs vary 3X in size, and the COI region varies about 5-fold

  2. The ITS region has become the de facto bar- coding region for ectomycorrhizal fungi for the following reasons: • Its easy to amplify in almost everything with general and fungal specific primers • In most taxa it has useful variation at approximately the morpho-species level • Paralogous copies are seldom a problem • There is a large existing database for it, including a recently well vouchered one (UNITE http://unite.ut.ee/)

  3. Problems with the ITS region for Ectomycorrhizal fungi • Database is not well populated for some groups (particularly among the Ascomycetes) • Does not align well beyond sub-generic comparisons • Occasionally too large or too divergent to amplify (Tulasnella, Cantharellus)

  4. Glomeromycota (AM fungi) • ITS seldom used for technical reasons (difficult to “nest”) • ITS may often have paralogous copies • 18S (and 25S) more often used, but clearly are not variable enough.

More Related