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The Simple Western is Here

The Simple Western is Here. Name Date. Western Blots are Tedious. Western Blots are Inconsistent. Day 1. Day 2. Meet Simon. Gel-free, blot-free, hands-free Simple Western Assays. Simon Gives You More. Ease of Use. Walk away assay convenience Fully analyzed data. Automation.

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The Simple Western is Here

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  1. The Simple Western is Here Name Date

  2. Western Blots are Tedious

  3. Western Blots are Inconsistent Day 1 Day 2

  4. Meet Simon Gel-free, blot-free, hands-free Simple Western Assays

  5. Simon Gives You More Ease of Use • Walk away assay convenience • Fully analyzed data Automation • Automated start to finish • Protocol standardization • Accurate quantitation • Greater reproducibility Higher Data Quality

  6. Simple Westerns are Easy Prepare samples Start Simon Results automatically

  7. Get More Information Ladder MCF7 MCF7 MCF7 MCF7 MCF7 MCF7 MCF7 MCF7 MCF7 MCF7 Traditional Western Blot Simple Western

  8. More Quantitative

  9. T a r g e t P r o t e i n R e p r o d u c i b i l i t y 1 4 0 0 0 1 2 0 0 0 1 0 0 0 0 8 0 0 0 C V = 5 . 2 % C V = 1 1 . 5 % C V = 1 2 . 0 % C V = 7 . 3 % C V = 9 . 7 % 6 0 0 0 4 0 0 0 2 0 0 0 0 M o n d a y T u e s d a y W e d n e s d a y T h u r s d a y A v e r a g e N i c k N a n c y T i m J e s s i c a E x p e r i m e n t s ( n = 1 1 r e p l i c a t e s ) More Reproducible Signal

  10. Global Standardization

  11. How Simple Westerns Work Separate Immobilize Immunoprobe Quantitate Signal

  12. Limits of Detection 2.0 mg/mL 0.016 0.008 0.125 0.064 0.032 0.25 1.0 0.5 250 2.0 mg/mL 0.016 0.008 Ladder Ladder 0.125 0.064 0.032 0.25 1.0 0.5 150 KDa 100 75 50 37 25 20 15 10 Simple Western Western Blot

  13. Wide Dynamic Range of Detection 3.0 mg/mL 0.0003 0.0001 Ladder 0.003 0.001 0.33 0.11 0.03 0.01 1.0 KDa

  14. Multiplexing Capabilities Ladder Rep 1 Rep 3 Rep 2 Rep 4 AKT2 Thioredoxin Courtesy of Senior Scientist pharmaceutical evaluator

  15. Comprehensive Pathway Validation

  16. Monitoring Biological Response MW - + - + MW Courtesy of Senior Scientist pharmaceutical evaluator Total STAT5 Phospho-STAT5

  17. Simple Westerns Offer More

  18. Complete Solution

  19. Powering Protein Research Signaling Purity Characterization Aggregation Imaging

  20. Supplemental Slides

  21. How the Simple Western Works

  22. Simple Western Assay Specs *Assay specifications apply only to the stated sizing range. However, proteins up to 200 kDa can be detected.

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