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An employment of flow cytometry in the biosystematics of the genus Galeobdolon

An employment of flow cytometry in the biosystematics of the genus Galeobdolon. Radka Rosenbaumová. Genus Galeobdolon. x = 9 2x = 18 4x = 36 3x = 27. Genus Galeobdolon. G. luteum. G. flavidum. 2n = 2x = 18. Genus Galeobdolon. G. montanum. 2n = 4x = 36. Genus Galeobdolon.

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An employment of flow cytometry in the biosystematics of the genus Galeobdolon

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  1. An employment of flow cytometry in the biosystematics of the genus Galeobdolon Radka Rosenbaumová

  2. Genus Galeobdolon x = 9 2x = 18 4x = 36 3x = 27

  3. Genus Galeobdolon G. luteum G. flavidum 2n = 2x = 18

  4. Genus Galeobdolon G. montanum 2n = 4x = 36

  5. Genus Galeobdolon G. argentatum 2n = 4x = 36

  6. Genus Galeobdolon G. luteum G. endtmannii G. montanum 4x 2x 2n = 2x = 18

  7. G. luteum ------- G. montanum ........ G. flavidum Genus Galeobdolon Distribution G. argentatum G. endtmannii

  8. 500 Standard 750 4x 400 Standard 600 2x 300 200 450 100 300 0 G. luteum G. flavidum 0 250 500 750 1000 G. montanum G. argentatum 150 0 0 250 500 750 1000 Ploidy level estimation Standard: Glycine max cv. Polanka, (2C = 2.5 pg)

  9. 500 2x 3x 400 4x 300 200 100 0 0 50 100 150 200 250 Ploidy level estimation Triploids

  10. Ploidy level estimation Triploids

  11. Ploidy level estimation Sympatric occurence 12% G. luteum G. montanum G. argentatum

  12. Ploidy level estimation G. endtmannii

  13. Ploidy level estimation G. endtmannii G. luteum G. montanum 2x 4x 4x 2x 2x + 4x

  14. Nuclear genome size determination

  15. Species 2C DNA content ± SE (pg) 1C DNA content (pg / Mbp) Genome size (pg) G. luteum 3.27 ± 0.002 1.63 / 1597 1.63 4.8 „G. endtmannii“ 3.28 ± 0.02 1.64 / 1607 1.64 G. flavidum 3.41 ± 0.02 1.70 / 1666 1.70 G. montanum 6.33 ± 0.04 3.17 / 3107 1.58 G. argentatum 6.36 ± 0.04 3.18 / 3116 1.59 4.9 „G. montanum“ 6.59 ± 0.04 3.29 / 3224 1.65 „G. endtmannii“ 6.41 ± 0.03 3.20 / 3136 1.60 Triploids 4.85 ± 0.04 2.43 / 2381 1.62 4.85 Nuclear genome size determination

  16. Species 2C DNA content ± SE (pg) 1C DNA content (pg / Mbp) Genome size (pg) G. luteum 3.27 ± 0.002 1.63 / 1597 1.63 „G. endtmannii“ 3.28 ± 0.02 1.64 / 1607 1.64 G. flavidum 3.41 ± 0.02 1.70 / 1666 1.70 G. montanum 6.33 ± 0.04 3.17 / 3107 1.58 G. argentatum 6.36 ± 0.04 3.18 / 3116 1.59 „G. montanum“ 6.59 ± 0.04 3.29 / 3224 1.65 „G. endtmannii“ 6.41 ± 0.03 3.20 / 3136 1.60 Triploids 4.85 ± 0.04 2.43 / 2381 1.62 Nuclear genome size determination

  17. 1250 G.l. G.f. 1000 750 500 250 0 0 250 500 750 1000 Nuclear genome size determination G. luteum / G. flavidum

  18. Species 2C DNA content ± SE (pg) 1C DNA content (pg / Mbp) Genome size (pg) G. luteum 3.27 ± 0.002 1.63 / 1597 1.63 „G. endtmannii“ 3.28 ± 0.02 1.64 / 1607 1.64 G. flavidum 3.41 ± 0.02 1.70 / 1666 1.70 G. montanum 6.33 ± 0.04 3.17 / 3107 1.58 G. argentatum 6.36 ± 0.04 3.18 / 3116 1.59 „G. montanum“ 6.59 ± 0.04 3.29 / 3224 1.65 3.7 % „G. endtmannii“ 6.41 ± 0.03 3.20 / 3136 1.60 Triploids 4.85 ± 0.04 2.43 / 2381 1.62 Nuclear genome size determination

  19. 900 „G.m“ 720 G.m. 540 360 180 0 0 250 500 750 1000 Nuclear genome size determination G. argentatum X G. montanum

  20. Nuclear genome size determination G. argentatum X G. montanum

  21. Conclusions 3x Sympatric occurence G. endtmannii G. flavidum G. montanum X G. argentatum

  22. An employment of flow cytometry in the biosystematics of the genus Galeobdolon

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